[Characterization of the cellular immune response induced by Rv2626c].

作者信息

Li Guo, Fu Hong, Gao Yunfei, Feng Youwei, Li Jing, Chen Chao, Zhong Dan, Chen Xiang, Yin Yuelan, Jiao Xin'an

机构信息

Jiangsu Key Laboratory of Zoonoses, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agri-fod Safety and Quality, Ministry of Agriculture and Rural Affairs, Joint International Research Laboratory of Agriculture and Agri-product Safety of Ministry of Education, Yangzhou University, Yangzhou 225009, Jiangsu, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2023 Jul 25;39(7):2644-2655. doi: 10.13345/j.cjb.230020.

DOI:10.13345/j.cjb.230020

PMID:37584121

Abstract

Nearly a quarter of the world's population is infected with and remains long-term asymptomatic infection. Rv2626c is a latent infection-related protein regulated by DosR of . In this study, the Rv2626c protein was prokaryotically expressed and purified, and its immunobiological characteristics were analyzed using RAW264.7 cells and mice as infection models. SDS-PAGE and Western blotting analysis showed that the Rv2626c-His fusion protein was mainly expressed in soluble form and specifically reacted with the rabbit anti-H37RV polyclonal serum. In addition, we found that the Rv2626c protein bound to the surface of RAW264.7 macrophages and up-regulated the production of NO. Moreover, the Rv2626c protein significantly induced the production of pro-inflammatory cytokines IFN-γ, TNF-α, IL-6 and MCP-1, and induced strong Th1-tendency immune response. These results may help to reveal the pathogenic mechanism of and facilitate the development of new tuberculosis vaccine.

摘要