Faculty of Physics and Applied Computer Science, AGH University of Krakow, Krakow, Poland.
Institute of Zoology and Biomedical Research, Jagiellonian University, Krakow, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 15;303:123230. doi: 10.1016/j.saa.2023.123230. Epub 2023 Aug 2.
Glioblastoma multiforme (GBM) is the most common and devastating primary brain tumor among adults. It is highly lethal disease, as only 25% of patients survive longer than 1 year and only 5% more than 5 years from the diagnosis. To search for the new, more effective methods of treatment, the understanding of mechanisms underlying the process of tumorigenesis is needed. The new light on this problem may be shed by the analysis of biochemical anomalies of tissues affected by tumor growth. Therefore, in the present work, we applied the Fourier transform infrared (FTIR) and Raman microspectroscopy to evaluate changes in the distribution and structure of biomolecules appearing in the rat brain as a result of glioblastoma development. In turn, synchrotron X-ray fluorescence microscopy was utilized to determine the elemental anomalies appearing in the nervous tissue. To achieve the assumed goals of the study animal models of GBM were used. The rats were subjected to the intracranial implantation of glioma cells with different degree of invasiveness. For spectroscopic investigation brain slices taken from the area of cancer cells administration were used. The obtained results revealed, among others, the decrease content of lipids and compounds containing carbonyl groups, compositional and structural changes of proteins as well as abnormalities in the distribution of low atomic number elements within the region of tumor.
多形性胶质母细胞瘤（GBM）是成年人中最常见且最具破坏性的原发性脑肿瘤。它是一种高度致命的疾病，只有 25%的患者在诊断后 1 年以上存活，只有 5%的患者存活时间超过 5 年。为了寻找新的、更有效的治疗方法，需要了解肿瘤发生过程中的机制。通过分析受肿瘤生长影响的组织的生化异常，可能会对这个问题有新的认识。因此，在本工作中，我们应用傅里叶变换红外（FTIR）和拉曼微光谱技术来评估大鼠脑内生物分子分布和结构的变化，这些变化是由于胶质母细胞瘤的发展而出现的。反过来，同步辐射 X 射线荧光显微镜被用来确定神经组织中出现的元素异常。为了实现研究的预期目标，我们使用了 GBM 的动物模型。这些大鼠接受了具有不同侵袭程度的胶质瘤细胞的颅内植入。为了进行光谱研究，使用了取自癌细胞给药区域的脑切片。获得的结果表明，除其他外，脂质和含有羰基基团的化合物含量降低，蛋白质的组成和结构发生变化，以及肿瘤区域内低原子数元素分布的异常。
