刺山柑（白花菜科）提取物调节氧化应激相关基因表达，恢复抗氧化酶活性，并预防二肾一夹（2K1C）大鼠肾脏和心脏的氧化应激。

Bark (Capparidaceae) Extract Modulates Oxidative Stress-Related Gene Expression, Restores Antioxidant Enzymes, and Prevents Oxidative Stress in the Kidney and Heart of 2K1C Rats.

作者信息

Jahan Ishrat, Saha Proma, Eysha Chisty Tammana Tabassum, Mitu Kaniz Fatima, Chowdhury Faizul Islam, Ahmed Khondoker Shahin, Hossain Hemayet, Khan Ferdous, Subhan Nusrat, Alam Md Ashraful

机构信息

Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh.

Chemical Research Division, BCSIR Laboratories, Dhaka, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka-1205, Bangladesh.

出版信息

Evid Based Complement Alternat Med. 2023 Aug 9;2023:4720727. doi: 10.1155/2023/4720727. eCollection 2023.

DOI:10.1155/2023/4720727

PMID:37593003

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10432060/

Abstract

OBJECTIVE

is a medicinal plant, which contains a wide range of polyphenolic and bioactive compounds. The aim of the study was to evaluate the renal-protective activity of in two-kidney, one-clip (2K1C) rats.

METHODS

In this study, the ethanol extract of bark at a dose of 100 mg/kg was orally used to treat 2K1C rats for four weeks. At the end of the experiment, all rats were sacrificed and tissue samples were collected for further biochemical and histological assessments.

RESULTS

This investigation showed that treatment prevented the kidney dysfunction in 2K1C rats. Uric acid and creatinine concentration and CK-MB activities increased in 2K1C rats which were normalized by . 2K1C rats also showed increased oxidative stress, depicted by the elevated level of MDA, NO, and APOP in plasma and tissues. Oxidative stress parameters declined in 2K1C rats by the treatment of . These results could be attributed to the restoration of antioxidant enzyme activities such as catalase and SOD. extracts also upregulated antioxidant gene expression in the kidneys of 2K1C rats. Moreover, several anti-inflammatory genes were suppressed by treatment in 2K1C rats. Furthermore, fibrosis and collagen deposition in the kidneys were also lowered by the treatment of the extract.

CONCLUSION

The experimental data suggest that the extract protected renal damage and oxidative stress, probably by restoring antioxidant enzymes activities in 2K1C rats.

摘要

目的

[植物名称]是一种药用植物，含有多种多酚类和生物活性化合物。本研究的目的是评估[植物名称]对二肾一夹（2K1C）大鼠的肾脏保护活性。

方法

在本研究中，以100mg/kg的剂量口服[植物名称]树皮的乙醇提取物，对2K1C大鼠进行为期四周的治疗。实验结束时，处死所有大鼠并收集组织样本，进行进一步的生化和组织学评估。

结果

本研究表明，[植物名称]治疗可预防2K1C大鼠的肾功能障碍。2K1C大鼠的尿酸、肌酐浓度和CK-MB活性升高，而[植物名称]可使其恢复正常。2K1C大鼠还表现出氧化应激增加，表现为血浆和组织中MDA、NO和APOP水平升高。[植物名称]治疗可使2K1C大鼠的氧化应激参数下降。这些结果可能归因于过氧化氢酶和超氧化物歧化酶等抗氧化酶活性的恢复。[植物名称]提取物还上调了2K1C大鼠肾脏中的抗氧化基因表达。此外，[植物名称]治疗可抑制2K1C大鼠中的几种抗炎基因。此外，[植物名称]提取物治疗还降低了肾脏中的纤维化和胶原蛋白沉积。