淀粉纳米颗粒作为保加利亚乳杆菌 CICC 6097 冷冻保护中新的冰晶核。
Starch nanoparticles as a new ice crystal nucleator in Lactobacillus bulgaricus CICC 6097 cryoprotection.
机构信息
College of Biological Science & Biotechnology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China.
College of Biological Science & Biotechnology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China.
出版信息
Int J Biol Macromol. 2023 Nov 1;251:126395. doi: 10.1016/j.ijbiomac.2023.126395. Epub 2023 Aug 17.
This work aimed at studying the cryoprotective effect of starch nanoparticles (SNPs) as ice nucleators on Lactobacillus bulgaricus CICC 6097 and exploring the protective mechanism. SNPs with a diameter of 363 nm were obtained, which possessed special properties to enhance ice nucleation and blunt ice crystals edges, and can be wrapped around the surface of bacteria form a protective barrier, thus maintaining the integrity of cell membranes and improving the survivability of L. bulgaricus CICC 6097 from 36.09% to 75.56%. However, SNCs solution formed ice crystals with obvious spikes during freezing, which was harmful to bacteria survival. Therefore, SNPs addition is a possible strategy for increasing viable cell counts during freezing. To the best of our knowledge, this report is the first to demonstrate the cryoprotective effect of ice nucleator on lactic acid bacteria.
本研究旨在探讨淀粉纳米颗粒（SNPs）作为冰核剂对保加利亚乳杆菌（Lactobacillus bulgaricus CICC 6097）的抗冷冻保护作用，并探索其保护机制。获得了直径为 363nm 的 SNPs，其具有增强冰核形成和钝化冰晶边缘的特殊性质，并可以包裹在细菌表面形成保护屏障，从而保持细胞膜的完整性，提高保加利亚乳杆菌 CICC 6097 的存活率从 36.09%提高到 75.56%。然而，SNCs 溶液在冷冻过程中形成了具有明显尖峰的冰晶，这对细菌的存活不利。因此，添加 SNPs 是提高冷冻过程中活菌数的一种可行策略。据我们所知，本报告首次证明了冰核剂对乳酸菌的抗冷冻保护作用。
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