School of Environmental and Municipal Engineering, Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin Chengjian University, Tianjin 300384, China; School of Civil Engineering and Architecture, Taizhou University, Taizhou 318000, Zhejiang, China.
School of Civil Engineering and Architecture, Taizhou University, Taizhou 318000, Zhejiang, China.
Bioresour Technol. 2023 Nov;387:129609. doi: 10.1016/j.biortech.2023.129609. Epub 2023 Aug 18.
A novel integrated sulfur fixed-film activated sludge in SBR system (ISFAS-SBR) was proposed to treat the low C/N ratio municipal wastewater. The effluent total inorganic nitrogen (TIN) and PO-P decreased from 17 mg/L and 3.5 mg/L to 8.5 mg/L and 0.5 mg/L, and higher nitrogen removal efficiency was contributed by the autotrophic denitrification. Microbial response characteristics showed that catalase (CAT), reduced nicotinamide adenine dinucleotide (NADH) and extracellular polymeric substance (EPS) alleviated the oxidative stress of sulfur carrier to maintain cell activity, while metabolic activity analysis indicated that the electron transfer rate was enhanced to improve mixotrophic denitrification efficiency. Meanwhile, the increased key enzyme activities further facilitated nitrogen removal and sulfur oxidation process. Additionally, the microbial community, functional proteins and genes revealed a niche equilibrium of C, N, S metabolic bacteria. Sulfur autotrophic in-situ coupled SBR system enlarged a promising strategy for treatment of low C/N ratio municipal wastewater.
一种新型的 SBR 系统中固定化硫的活性污泥（ISFAS-SBR）被提出用于处理低 C/N 比的城市废水。出水中的总无机氮（TIN）和 PO-P 从 17mg/L 和 3.5mg/L 降低到 8.5mg/L 和 0.5mg/L，更高的脱氮效率归因于自养反硝化。微生物响应特性表明，过氧化氢酶（CAT）、还原型烟酰胺腺嘌呤二核苷酸（NADH）和胞外聚合物（EPS）缓解了硫载体的氧化应激，维持了细胞活性，而代谢活性分析表明，电子传递速率提高，从而提高了混合营养反硝化效率。同时，增加的关键酶活性进一步促进了氮去除和硫氧化过程。此外，微生物群落、功能蛋白和基因揭示了 C、N、S 代谢细菌的小生境平衡。硫自养原位耦合 SBR 系统为处理低 C/N 比城市废水提供了一种很有前途的策略。
