Reinikovaite Viktorija, Matulevicius Matas, Elsakova Alexandra, Drobysh Maryia, Liustrovaite Viktorija, Luksa Algirdas, Jafarov Ali, Slibinskas Rimantas, Ramanavicius Arunas, Baradoke Ausra
Department of Physical Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko str. 24, 03225 Vilnius, Lithuania.
Department of Physical Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko str. 24, 03225 Vilnius, Lithuania; Institute of Technology, Nooruse 1, 50411 Tartu, Estonia.
Sci Total Environ. 2023 Dec 10;903:166447. doi: 10.1016/j.scitotenv.2023.166447. Epub 2023 Aug 19.
In this study, we are reporting a novel electrochemical capacitance spectroscopy (ECS) platform designed for the sensitive and label-free detection of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus spike protein (anti-rS) in diluted blood serum. The determination of anti-rS is crucial for identification individuals who have been infected by SARS-CoV-2 virus and may have acquired immunity. The rS protein was immobilized on a screen-printed carbon electrode, which was incubated in diluted blood serum containing anti-rS antibodies. Label-free ECS was applied for the determination of interaction between immobilized rS and free-standing anti-rS. Here reported bioanalytical platform demonstrated high sensitivity and specificity in detecting anti-rS, achieving a limit of detection of 4.38 nM. This versatile platform could be further enhanced by applying various electrode materials and adapting this platform to detect antibodies against some other proteins. Our findings have significant implications for the development of affordable, scalable biosensing platforms capable to provide rapid and accurate public health screening and monitoring, particularly in the context of the coronavirus disease 2019 (COVID-19) pandemic.
在本研究中，我们报告了一种新型电化学电容光谱（ECS）平台，该平台设计用于灵敏且无标记地检测稀释血清中针对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）病毒刺突蛋白（抗rS）的抗体。抗rS的测定对于识别感染了SARS-CoV-2病毒且可能已获得免疫力的个体至关重要。将rS蛋白固定在丝网印刷碳电极上，该电极在含有抗rS抗体的稀释血清中孵育。采用无标记ECS来测定固定化rS与游离抗rS之间的相互作用。此处报道的生物分析平台在检测抗rS方面表现出高灵敏度和特异性，检测限达到4.38 nM。通过应用各种电极材料并使该平台适用于检测针对其他一些蛋白质的抗体，这个多功能平台可以得到进一步提升。我们的研究结果对于开发经济实惠、可扩展的生物传感平台具有重要意义，这些平台能够提供快速准确的公共卫生筛查和监测，特别是在2019年冠状病毒病（COVID-19）大流行的背景下。
