Department of Anesthesiology, The First Hospital of Hebei Medical University, Shijiazhuang, China.
Operating Center, The First Hospital of Hebei Medical University, Shijiazhuang, China.
Chem Biol Drug Des. 2023 Nov;102(5):1176-1185. doi: 10.1111/cbdd.14324. Epub 2023 Aug 21.
The present study was conducted to determine the protective effect of Dexmedetomidine (DEX) in myocardial ischemia-reperfusion injury in hyperlipidemic rats. Towards this, the effect of DEX was first evaluated on the infarct size and the histopathology of cardiac tissues using TTC and H and E staining, and it was found that DEX significantly improved the infarct size and architecture of the myocardial tissues following the I/R injury. DEX also showed significant improvement in various examined hemodynamic parameters (e.g., LVSP, and ± dp/dt ) in a dose-dependent manner. The lipid profile (LDL, VLDL, TC, TG, and HDL level) of the rats were also found significantly improved in DEX-treated rats. The level of various pro-inflammatory cytokines (IL-1β, IL-6, IL-10, IL-17, and TNF-α), cardiac injury (CK, CK-MB, Troponin I AST, ALT, and LDH), and oxidative stress (MDA, SOD, and GSH) biomarkers were also found to be restored near to the normal in DEX-treated group. It has been found that DEX also significantly reduces apoptosis of rat cardiomyocytes. In western blot analysis, DEX showed a significant reduction in the activation of NF-κB. In conclusion, our study demonstrated the protective effect of Dexmedetomidine in myocardial ischemia-reperfusion injury in hyperlipidemic rats possibly via amelioration of oxidative stress, and inflammation apoptosis.
本研究旨在探讨右美托咪定（DEX）对高脂血症大鼠心肌缺血再灌注损伤的保护作用。为此，首先通过 TTC 和 H&E 染色评估了 DEX 对梗死面积和心脏组织病理学的影响，结果发现 DEX 显著改善了 I/R 损伤后心肌组织的梗死面积和结构。DEX 还以剂量依赖的方式显著改善了各种检查的血流动力学参数（例如 LVSP 和±dp/dt）。DEX 治疗的大鼠血脂谱（LDL、VLDL、TC、TG 和 HDL 水平）也明显改善。各种促炎细胞因子（IL-1β、IL-6、IL-10、IL-17 和 TNF-α）、心脏损伤标志物（CK、CK-MB、肌钙蛋白 I AST、ALT 和 LDH）和氧化应激标志物（MDA、SOD 和 GSH）的水平在 DEX 治疗组也恢复到接近正常水平。还发现 DEX 还显著减少了大鼠心肌细胞的凋亡。在 Western blot 分析中，DEX 显示 NF-κB 的激活显著减少。综上所述，本研究表明 DEX 对高脂血症大鼠心肌缺血再灌注损伤具有保护作用，可能通过改善氧化应激和炎症凋亡。
