Qiu Guankai, Wang Qirong, Wang Quanying, Wang Tianye, Kang Zhichao, Zeng Ying, Yang Xiutao, Song Ningning, Zhang Shaoqing, Han Xuerong, Yu Hongwen
Key Laboratory of Wetland Ecology and Environment, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130102, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Key Laboratory of Wetland Ecology and Environment, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, 130102, China.
Environ Res. 2023 Nov 15;237(Pt 2):116976. doi: 10.1016/j.envres.2023.116976. Epub 2023 Aug 23.
Soil, as a heterogeneous body, is composed of different-sized aggregates. There is limited data available on the potential role of microplastics (MPs) in microbial properties at the soil aggregate level. In this study, changes in microbial construction and diversity in farmland bulk soil and aggregates induced by polyethylene MPs (PE-MPs) were investigated at a dose of 0.5% (w/w) through 16s rDNA sequencing and enzyme activity measurements of different particle size aggregates in incubated soil. The presence of low-dose PE-MPs increased the proportion of >1 mm soil aggregates fraction, and decreased soil available nitrogen and available phosphorus in bulk soils. Furthermore, low-dose PE-MPs increased bacterial richness and diversity in 1-0.5 and < 0.25 mm fractions and decreased operational taxonomic unit, abundance-based coverage estimator, and Chao1 indices in bulk soil and >1 mm fractions. The levels of predicted functional genes taking part in the biodegradation and metabolism of exogenous substances also increased. At the phylum level, PE-MPs changed the proportion of Proteobacteria and Actinobacteria. The variations in soil aggregate properties were significantly correlated with the bacterial communities' composition and diversity. This study deepens our perception of the soil microenvironment, microbial community composition, and diversity in response to PE-MPs.
土壤作为一种非均质体，由不同大小的团聚体组成。关于微塑料（MPs）在土壤团聚体水平上对微生物特性的潜在作用，现有数据有限。在本研究中，通过16s rDNA测序和对培养土壤中不同粒径团聚体的酶活性测量，研究了剂量为0.5%（w/w）的聚乙烯微塑料（PE-MPs）对农田原状土壤和团聚体中微生物结构和多样性的影响。低剂量PE-MPs的存在增加了>1mm土壤团聚体部分的比例，并降低了原状土壤中的有效氮和有效磷。此外，低剂量PE-MPs增加了1-0.5mm和<0.25mm部分的细菌丰富度和多样性，并降低了原状土壤和>1mm部分的操作分类单元、基于丰度的覆盖率估计值和Chao1指数。参与外源物质生物降解和代谢的预测功能基因水平也有所增加。在门水平上，PE-MPs改变了变形菌门和放线菌门的比例。土壤团聚体性质的变化与细菌群落的组成和多样性显著相关。本研究加深了我们对土壤微环境、微生物群落组成以及对PE-MPs响应的多样性的认识。
