Song Wenwen, Dai Mingming, Shi Qianqian, Liang Chen, Duan Fangmeng, Zhao Honghai
Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China.
Life (Basel). 2023 Aug 16;13(8):1758. doi: 10.3390/life13081758.
The potato rot nematode () is one of the most destructive pests in the production of tuber crops, resulting in severely decreased yields and inferior product quality. In 2021, a great number of nematodes were detected in the roots of , a weed that is harmful to crop growth, in Qingdao, Shandong Province, China. The present study was undertaken to characterize and identify the nematodes isolated from through morphological identification and molecular approaches. Their morphological characteristics were highly consistent with the descriptions of Thorne, 1945. The nematodes collected from were identified as haplotype B using D1/D2 and sequence characterized amplified region (SCAR) primers. PCR-ITS-RFLP analysis was conducted to monitor intraspecific variations. In addition, the phylogenetic analysis of the internal transcribed spacer (ITS) demonstrated that this population was clustered in haplotype B, supported by a 100% bootstrap value. Another assay, in which was inoculated with a mixture of the life stages of , was performed. This assay showed that exhibited a high susceptibility to in pots. This is the first record of parasitizing in China, and it is of great importance because could be a potential reservoir for in the field.
马铃薯腐烂线虫（）是块茎作物生产中最具破坏性的害虫之一，会导致产量严重下降和产品质量低劣。2021年，在中国山东省青岛市，在对作物生长有害的杂草的根部检测到大量线虫。本研究旨在通过形态学鉴定和分子方法对从分离出的线虫进行特征描述和鉴定。它们的形态特征与1945年索恩对的描述高度一致。使用D1/D2和序列特征扩增区域（SCAR）引物，将从采集的线虫鉴定为单倍型B。进行了PCR-ITS-RFLP分析以监测种内变异。此外，对内部转录间隔区（ITS）的系统发育分析表明，该线虫群体聚集在单倍型B中，自展值为100%。进行了另一项试验，用的不同生活阶段的混合物接种。该试验表明，在花盆中对表现出高度敏感性。这是在中国寄生的首次记录，并且非常重要，因为可能是田间的潜在宿主。
