GRP78 在非小细胞肺癌组织中过表达，并与鳞状细胞癌中高 VEGF 表达相关：一项初步研究。

GRP78 is Overexpressed in Non-small Cell Lung Cancer Tissues and is Associated with High VEGF Expression in Squamous Cell Carcinoma: A Pilot Study.

机构信息

Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid 22110, Jordan.

Department of Pathology and Microbiology, Jordan University of Science and Technology, Irbid 22110, Jordan.

出版信息

Curr Cancer Drug Targets. 2023;23(10):805-816. doi: 10.2174/1568009623666230418111020.

DOI:10.2174/1568009623666230418111020

PMID:37638596

Abstract

BACKGROUND

Neovascularization is essential for the growth and progression of tumor tissues. GRP78 is frequently overexpressed in various cancers and has been suggested as a proangiogenic factor.

PURPOSE

This study aimed to investigate the expression levels of GRP78 and to test for significant relationships with the angiogenic markers, VEGF, and CD31.

METHODS

In this study, paraffin-embedded NSCLC tissue samples (71 adenocarcinomas and 23 squamous cell carcinoma) were retrospectively collected from 94 patients with NSCLC. The expressions of VEGF, CD31, and GRP78 were determined by immunohistochemistry.

RESULTS

High expression levels of VEGF and GRP78 were observed in 65 and 74 cases, respectively. Thirty-six patients expressed high CD31 levels. Adenocarcinomas expressed higher levels of the three proteins than squamous cell carcinomas (p-value < 0.05). Moreover, a statistically significant association was found between the expression levels of VEGF and CD31 (p-value = 0.001) and VEGF and GRP78 (p-value=0.028).

CONCLUSION

GRP78 overexpression was revealed in most of the investigated samples. The positive association between VEGF and GRP78 may indicate the proangiogenic role of GRP78 in lung cancer. Moreover, the positive association between VEGF and CD31 expression levels suggests that VEGF may cooperate with CD31 to promote angiogenesis in NSCLC.

摘要

背景

新生血管化对于肿瘤组织的生长和进展至关重要。GRP78 在各种癌症中经常过表达，并被认为是一种促血管生成因子。

目的

本研究旨在研究 GRP78 的表达水平，并检测其与血管生成标志物 VEGF 和 CD31 之间的显著关系。

方法

本研究回顾性收集了 94 例 NSCLC 患者的石蜡包埋 NSCLC 组织样本（71 例腺癌和 23 例鳞状细胞癌）。通过免疫组织化学法检测 VEGF、CD31 和 GRP78 的表达。

结果

VEGF 和 GRP78 的高表达水平分别在 65 例和 74 例中观察到。36 例患者表达高水平的 CD31。腺癌中这三种蛋白的表达水平均高于鳞状细胞癌（p 值 < 0.05）。此外，还发现 VEGF 和 CD31（p 值=0.001）以及 VEGF 和 GRP78（p 值=0.028）之间存在统计学显著关联。