• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

产超广谱β-内酰胺酶和不产超广谱β-内酰胺酶的尿道分离株在克隆分布、毒力基因含量和系统发育群方面存在差异。

ESBL-Producing and Non-ESBL-Producing Isolates from Urinary Tract Differ in Clonal Distribution, Virulence Gene Content and Phylogenetic Group.

作者信息

Fang Yewei, Tao Shuan, Chen Huimin, Xu Yao, Chen Luyan, Liang Wei

机构信息

Department of Clinical Laboratory, The First Affiliated Hospital of Ningbo University, Ningbo, People's Republic of China.

School of Medicine, Jiangsu University, Zhejiang, People's Republic of China.

出版信息

Infect Drug Resist. 2023 Aug 23;16:5563-5571. doi: 10.2147/IDR.S423860. eCollection 2023.

DOI:10.2147/IDR.S423860
PMID:37641799
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10460598/
Abstract

PURPOSE

The objectives of this study are to determine the differences in clonality, virulence gene (VG) content and phylogenetic group between non extended-spectrum beta-lactamase-producing (non-ESBL-EC) and ESBL-EC isolates from urine.

PATIENTS AND METHODS

This study characterized a total of 100 clinical isolates consecutively obtained from the inpatients hospitalized in The First Affiliated Hospital of Ningbo University in China by polymerase-chain reaction (PCR).

RESULTS

Phylogenetic group B2 was found to be the most prevalent in both ESBL-EC and non-ESBL-EC group. Among 100 clinical isolates, the count of acquired virulence genes in group B2 was found to be significantly higher than that in group A, B1, and D ( <0.001). Additionally, the presence of content within virulence genes (the total number of virulence genes detected per isolate) in B2 of non-ESBL-EC and ESBL-EC showed a significant difference (<0.001). ST131 was detected exclusively in ESBL-EC, while ST95 and ST73 were the main sequence types in non-ESBL-EC.

CONCLUSION

Our study demonstrated the different distribution of MLST, phylogenetic group in ESBL-EC and non-ESBL-EC group. The inverse association between beta-lactamase resistance and VG content performed in this study should get a lot more attention. At the same time, we should also be wary of the appearance of non-ESBL-EC isolates of group B2 harboring more virulence genes which will lead to high pathogenicity.

摘要

目的

本研究的目的是确定尿中分离出的非产超广谱β-内酰胺酶（非ESBL-EC）和ESBL-EC菌株在克隆性、毒力基因（VG）含量和系统发育群方面的差异。

患者和方法

本研究通过聚合酶链反应（PCR）对连续从中国宁波大学附属第一医院住院患者中获得的100株临床分离株进行了特征分析。

结果

发现系统发育群B2在ESBL-EC组和非ESBL-EC组中最为普遍。在100株临床分离株中，发现B2组中获得的毒力基因数量显著高于A组、B1组和D组（<0.001）。此外，非ESBL-EC和ESBL-EC的B2组中毒力基因内的含量（每株分离株检测到的毒力基因总数）存在显著差异（<0.001）。ST131仅在ESBL-EC中检测到，而ST95和ST73是非ESBL-EC中的主要序列类型。

结论

我们的研究证明了ESBL-EC组和非ESBL-EC组中多位点序列分型（MLST）、系统发育群的不同分布。本研究中β-内酰胺酶耐药性与VG含量之间的负相关关系应得到更多关注。同时，我们也应警惕携带更多毒力基因的B2组非ESBL-EC分离株的出现，这将导致高致病性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9606/10460598/d6dbf2566819/IDR-16-5563-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9606/10460598/322eb1f64e31/IDR-16-5563-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9606/10460598/a73ed871d9f9/IDR-16-5563-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9606/10460598/2d28c8a2faf9/IDR-16-5563-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9606/10460598/18bb6543811a/IDR-16-5563-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9606/10460598/7e44e8ba9c3e/IDR-16-5563-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9606/10460598/d6dbf2566819/IDR-16-5563-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9606/10460598/322eb1f64e31/IDR-16-5563-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9606/10460598/a73ed871d9f9/IDR-16-5563-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9606/10460598/2d28c8a2faf9/IDR-16-5563-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9606/10460598/18bb6543811a/IDR-16-5563-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9606/10460598/7e44e8ba9c3e/IDR-16-5563-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9606/10460598/d6dbf2566819/IDR-16-5563-g0006.jpg

相似文献

1
ESBL-Producing and Non-ESBL-Producing Isolates from Urinary Tract Differ in Clonal Distribution, Virulence Gene Content and Phylogenetic Group.产超广谱β-内酰胺酶和不产超广谱β-内酰胺酶的尿道分离株在克隆分布、毒力基因含量和系统发育群方面存在差异。
Infect Drug Resist. 2023 Aug 23;16:5563-5571. doi: 10.2147/IDR.S423860. eCollection 2023.
2
Extended-spectrum beta-lactamase (ESBL)-producing and non-ESBL-producing Escherichia coli isolates causing bacteremia in the Netherlands (2014 - 2016) differ in clonal distribution, antimicrobial resistance gene and virulence gene content.荷兰产Extended-spectrum beta-lactamase (ESBL)和非产 ESBL 的大肠杆菌引起菌血症的分离株（2014-2016 年）在克隆分布、抗菌药物耐药基因和毒力基因组成方面存在差异。
PLoS One. 2020 Jan 14;15(1):e0227604. doi: 10.1371/journal.pone.0227604. eCollection 2020.
3
Community Fecal Carriage and Molecular Epidemiology of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing from Healthy Children in the Central South China.中国中南地区健康儿童产超广谱β-内酰胺酶和碳青霉烯酶细菌的社区粪便携带情况及分子流行病学研究
Infect Drug Resist. 2022 Apr 6;15:1601-1611. doi: 10.2147/IDR.S357090. eCollection 2022.
4
First report of the new emerging global clone ST1193 among clinical isolates of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli from Germany.德国产超广谱β-内酰胺酶（ESBL）大肠埃希菌临床分离株中新出现的全球克隆 ST1193 的首次报告。
J Glob Antimicrob Resist. 2019 Jun;17:305-308. doi: 10.1016/j.jgar.2019.01.014. Epub 2019 Jan 22.
5
Dominance of CTX-M-type extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from patients with community-onset and hospital-onset infection in China.中国社区获得性感染和医院获得性感染患者中分离出的产CTX-M型超广谱β-内酰胺酶（ESBL）大肠埃希菌的优势情况。
PLoS One. 2014 Jul 1;9(7):e100707. doi: 10.1371/journal.pone.0100707. eCollection 2014.
6
Emergence and spread of B2-ST131-O25b, B2-ST131-O16 and D-ST405 clonal groups among extended-spectrum-β-lactamase-producing Escherichia coli in Japan.产超广谱β-内酰胺酶大肠埃希菌中 B2-ST131-O25b、B2-ST131-O16 和 D-ST405 克隆群的出现和传播。
J Antimicrob Chemother. 2012 Nov;67(11):2612-20. doi: 10.1093/jac/dks278. Epub 2012 Jul 26.
7
High prevalence of B2-ST131 clonal group among extended-spectrum β-lactamase-producing Escherichia coli isolated from bloodstream infections in Quito, Ecuador.厄瓜多尔基多血流感染中产超广谱β-内酰胺酶大肠埃希菌中 B2-ST131 克隆群的高流行率。
J Glob Antimicrob Resist. 2019 Dec;19:216-221. doi: 10.1016/j.jgar.2019.04.019. Epub 2019 May 8.
8
National survey of Escherichia coli causing extraintestinal infections reveals the spread of drug-resistant clonal groups O25b:H4-B2-ST131, O15:H1-D-ST393 and CGA-D-ST69 with high virulence gene content in Spain.西班牙一项关于引起肠外感染的大肠杆菌的全国性调查揭示了具有高毒力基因含量的耐药克隆群 O25b:H4-B2-ST131、O15:H1-D-ST393 和 CGA-D-ST69 的传播。
J Antimicrob Chemother. 2011 Sep;66(9):2011-21. doi: 10.1093/jac/dkr235. Epub 2011 Jun 13.
9
CTX-M-15-D-ST648 Escherichia coli from companion animals and horses: another pandemic clone combining multiresistance and extraintestinal virulence?来自伴侣动物和马匹的CTX-M-15-D-ST648大肠杆菌：又是一个兼具多重耐药性和肠外致病性的大流行克隆株？
J Antimicrob Chemother. 2014 May;69(5):1224-30. doi: 10.1093/jac/dkt516. Epub 2014 Jan 6.
10
Multidrug resistance dissemination by extended-spectrum β-lactamase-producing Escherichia coli causing community-acquired urinary tract infection in the Central-Western Region, Brazil.产超广谱β-内酰胺酶大肠埃希菌引起巴西中西部地区社区获得性尿路感染的耐药性传播。
J Glob Antimicrob Resist. 2016 Sep;6:1-4. doi: 10.1016/j.jgar.2016.02.003. Epub 2016 Mar 26.

本文引用的文献

1
Characteristics of Escherichia coli Urine Isolates and Risk Factors for Secondary Bloodstream Infections in Patients with Urinary Tract Infections.大肠埃希菌尿分离株的特征和尿路感染患者继发血流感染的危险因素。
Microbiol Spectr. 2022 Aug 31;10(4):e0166022. doi: 10.1128/spectrum.01660-22. Epub 2022 Jul 7.
2
Antimicrobial Resistance and Molecular Epidemiology of From Bloodstream Infection in Shanghai, China, 2016-2019.2016 - 2019年中国上海血流感染的抗菌药物耐药性与分子流行病学
Front Med (Lausanne). 2022 Jan 10;8:803837. doi: 10.3389/fmed.2021.803837. eCollection 2021.
3
Clonal/subclonal changes and accumulation of CTX-M-type β-lactamase genes in fluoroquinolone-resistant Escherichia coli ST131 and ST1193 strains isolated during the past 12 years, Japan.
过去 12 年间，日本分离的耐氟喹诺酮大肠埃希菌 ST131 和 ST1193 株中 CTX-M 型β-内酰胺酶基因的克隆/亚克隆变化和积累。
J Glob Antimicrob Resist. 2021 Dec;27:150-155. doi: 10.1016/j.jgar.2021.08.015. Epub 2021 Sep 10.
4
High Prevalence of Extended-Spectrum Beta-Lactamases in Strains Collected From Strictly Defined Community-Acquired Urinary Tract Infections in Adults in China: A Multicenter Prospective Clinical Microbiological and Molecular Study.中国成人严格定义的社区获得性尿路感染分离菌株中超广谱β-内酰胺酶的高流行率：一项多中心前瞻性临床微生物学和分子研究
Front Microbiol. 2021 Jul 7;12:663033. doi: 10.3389/fmicb.2021.663033. eCollection 2021.
5
Emergence of ST131 Causing Urinary Tract Infection in Western Asia: A Systematic Review and Meta-Analysis.西亚引起尿路感染的 ST131 菌株的出现：系统评价和荟萃分析。
Microb Drug Resist. 2020 Nov;26(11):1357-1364. doi: 10.1089/mdr.2019.0312. Epub 2020 May 7.
6
Distribution of virulence genes and phylogenetics of uropathogenic Escherichia coli among urinary tract infection patients in Addis Ababa, Ethiopia.埃塞俄比亚亚的斯亚贝巴尿路感染患者中产脲原性大肠埃希菌的毒力基因分布和系统发育分析。
BMC Infect Dis. 2020 Feb 7;20(1):108. doi: 10.1186/s12879-020-4844-z.
7
Direct medical burden of antimicrobial-resistant healthcare-associated infections: empirical evidence from China.抗菌药物耐药性相关的医疗保健相关性感染的直接医疗负担：来自中国的实证证据。
J Hosp Infect. 2020 Jun;105(2):295-305. doi: 10.1016/j.jhin.2020.01.003. Epub 2020 Jan 10.
8
Diversity and trends in population structure of ESBL-producing Enterobacteriaceae in febrile urinary tract infections in children in France from 2014 to 2017.2014 年至 2017 年法国儿童发热性尿路感染中产 ESBL 肠杆菌科细菌的人群结构多样性和趋势。
J Antimicrob Chemother. 2020 Jan 1;75(1):96-105. doi: 10.1093/jac/dkz423.
9
Treatment of urinary tract infections in the era of antimicrobial resistance and new antimicrobial agents.抗菌药物耐药性和新型抗菌药物时代的尿路感染治疗。
Postgrad Med. 2020 Apr;132(3):234-250. doi: 10.1080/00325481.2019.1680052. Epub 2019 Oct 24.
10
Phylogenic classification and virulence genes profiles of uropathogenic E. coli and diarrhegenic E. coli  strains isolated from community acquired infections.社区获得性感染分离的尿路致病性大肠杆菌和腹泻性大肠杆菌的系统发育分类和毒力基因特征。
PLoS One. 2019 Sep 12;14(9):e0222441. doi: 10.1371/journal.pone.0222441. eCollection 2019.