泰国东北部低地地区结直肠癌患者 MLH1 rs1800734 致病性变异体。
MLH1 rs1800734 Pathogenic Variant among Patients with Colorectal Cancer in the Lower Northeastern Region of Thailand.
机构信息
Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Rangsit Campus, Pathum Thani, Thailand.
Department of Medical Technology, Faculty of Allied Health Sciences, Pathumthani University, Pathum Thani, Thailand.
出版信息
Asian Pac J Cancer Prev. 2023 Aug 1;24(8):2911-2916. doi: 10.31557/APJCP.2023.24.8.2911.
BACKGROUND
The -93G > A (rs1800734) polymorphism within the core promoter region of MLH1 gene is associated with MLH1 CpG island hypermethylation. This polymorphism has recently been proposed as a low penetrance variant for colorectal cancer. Many published studies have evaluated the association between the MLH1 -93G > A polymorphism and colorectal cancer risk. However, the results remain conflicting rather than conclusive. The aim of this study was to assess the association between the MLH1 -93G > A polymorphism and the risk of colorectal cancer in patients with colorectal cancer in the lower northeastern region of Thailand.
METHODS
One hundred fifty one samples from colorectal cancer patients and 100 samples from healthy control group were analyzed. Genomic DNA was extracted from white blood cell of all samples. The real-time polymerase chain reaction (qPCR) was used to demonstrate genetic polymorphism of MLH1 rs1800734.
RESULTS
This study demonstrated that the frequency of MLH1 rs1800734 in patients with colorectal cancer was higher than healthy control group. The MLH1 rs1800734 polymorphism variant AA was associated with an increased risk of colorectal cancer (p < 0.05). The MLH1 polymorphism variant AA carriers presented 1.36-folds high risk of colorectal cancer and the alcohol consumption was linked to their likelihood of developing colorectal cancer and their tumor's grade.
CONCLUSION
This study showed that MLH1 rs1800734 genotype AA was associated with colorectal cancer risk in the lower northeastern region of Thailand.
背景
MLH1 基因核心启动子区域的-93G > A（rs1800734）多态性与 MLH1 CpG 岛甲基化有关。这种多态性最近被提议为结直肠癌的低外显率变体。许多已发表的研究评估了 MLH1-93G > A 多态性与结直肠癌风险之间的关联。然而，结果仍然存在争议，而不是结论性的。本研究旨在评估 MLH1-93G > A 多态性与泰国东北部地区结直肠癌患者结直肠癌风险之间的关联。
方法
分析了 151 例结直肠癌患者和 100 例健康对照组的样本。从所有样本的白细胞中提取基因组 DNA。实时聚合酶链反应（qPCR）用于显示 MLH1 rs1800734 的遗传多态性。
结果
本研究表明，结直肠癌患者 MLH1 rs1800734 的频率高于健康对照组。MLH1 rs1800734 多态性变体 AA 与结直肠癌风险增加相关（p < 0.05）。MLH1 多态性变体 AA 携带者患结直肠癌的风险增加 1.36 倍，饮酒与他们患结直肠癌和肿瘤分级的可能性有关。
结论
本研究表明，MLH1 rs1800734 基因型 AA 与泰国东北部地区结直肠癌的风险相关。
Zotero 插件