Arıkan Sedat, Guven Soner, Sehitoglu Muserref Hilal, Elmas Sait
Department of Ophthalmology, Kayseri City Training and Research Hospital, Muhsin Yazıcıoglu Aveniu, Seker Street, No:77, Kocasinan, 38038, Kayseri, Turkey.
Department of Medical Biochemistry, Canakkale Onsekiz Mart University School of Medicine, Canakkale, Turkey.
Int Ophthalmol. 2023 Dec;43(12):4451-4460. doi: 10.1007/s10792-023-02845-5. Epub 2023 Aug 29.
To investigate the inhibitory effect of topically administered azithromycin (AZM), and moxifloxacin (MXF) against tumor necrosis factor-α (TNF-α) production in a rat model of endotoxin-induced uveitis (EIU).
Thirty-six Wistar albino rats were divided into 6 equal groups. Groups 1, 2 and 3 were determined as sham, control group for topical AZM application and control group for topical MXF application, respectively. Sterile saline, topical AZM 1.5%, and topical MXF 0.5% were instilled 5 times daily for totally 6 days on both eyes of the rats in Group 4, Group 5, and Group 6, before and after inducing EIU by intravitreal injections of lipopolysaccharide, respectively. At 24 h after intravitreal injections, aqueous humor was collected from both eyes of each rat for the assessment of TNF-α concentration. Also, density of nuclear factor kappa B (NF-κB) in ciliary body, and the number of cells infiltrating the posterior segment of EIU rat eyes was assessed in one eye of each rat.
There was a significant reduction in mean aqueous humor concentration of TNF-α in EIU rats pretreated with topical AZM in comparison with those pretreated with sterile saline (139 ± 38.6 in Group 4 vs. 72 ± 12.6 in Group 5, p = 0.006). There was also a marked decrease in mean aqueous humor concentration of TNF-α in EIU rats pretreated with topical MXF (139 ± 38.6 in Group 4 vs.86.1 ± 35.5 in Group 6, p = 0.025). Also, evident suppressions were determined in mean density of NF-κB, and in mean number of cells in EIU rats pretreated either with topical AZM, or topical MXF.
Topically applied AZM or MXF may be beneficial in the suppression of TNF-α production in aqueous humor.
研究局部应用阿奇霉素（AZM）和莫西沙星（MXF）对内毒素诱导的葡萄膜炎（EIU）大鼠模型中肿瘤坏死因子-α（TNF-α）产生的抑制作用。
将36只Wistar白化大鼠分为6个相等的组。第1、2和3组分别确定为假手术组、局部应用AZM的对照组和局部应用MXF的对照组。在通过玻璃体内注射脂多糖诱导EIU之前和之后，分别对第4、5和6组大鼠的双眼每天滴注5次无菌生理盐水、1.5%的局部用AZM和0.5%的局部用MXF，共6天。在玻璃体内注射后24小时，从每只大鼠的双眼收集房水，用于评估TNF-α浓度。此外，在每只大鼠的一只眼睛中评估睫状体中核因子κB（NF-κB）的密度以及EIU大鼠眼后段浸润细胞的数量。
与用无菌生理盐水预处理的EIU大鼠相比，局部应用AZM预处理的EIU大鼠房水中TNF-α的平均浓度显著降低（第4组为139±38.6对比第5组为72±12.6，p = 0.006）。局部应用MXF预处理的EIU大鼠房水中TNF-α的平均浓度也显著降低（第4组为139±38.6对比第6组为86.1±35.5，p = 0.025）。此外，在局部应用AZM或局部应用MXF预处理的EIU大鼠中，NF-κB的平均密度和细胞的平均数量均有明显抑制。
局部应用AZM或MXF可能有助于抑制房水中TNF-α的产生。
