Luo Kun, Zhao Gonghua, Chen Mengfei, Tian Xueliang
Hunan Agricultural University, Changsha, Hunan, China.
Henan Engineering Research Center of Biological Pesticide & Fertilizer Development and Synergistic Application, Henan Institute of Science and Technology, Xinxiang, Henan, China.
Front Plant Sci. 2023 Aug 14;14:1241055. doi: 10.3389/fpls.2023.1241055. eCollection 2023.
It is well known that plant genotype can regulate phyllosphere fungi at the species level. However, little is known about how plant varieties shape the fungal communities in the phyllosphere. In this study, four types of maize varieties with various levels of resistances to were subjected to high-throughput sequencing to reveal the properties that influences the composition of phyllosphere fungal communities. The dominant fungi genera for all four maize varieties were at different relative abundances, followed by . Hierarchical clustering analysis, non-metric multidimensional scaling and similarity analysis confirmed that the fungal communities in the phyllosphere of the four varieties were significantly different and clustered into the respective maize variety they inhabited. The findings from Redundancy Analysis (RDA) indicated that both maize resistance and leaf chemical constituents, including nitrogen, phosphorus, tannins, and flavonoids, were the major drivers in determining the composition of phyllosphere fungal communities. Among these factors, maize resistance was found to be the most influential, followed by phosphorus. The co-occurrence network of the fungal communities in the phyllosphere of highly resistant variety had higher complexity, integrity and stability compared to others maize varieties. In a conclusion, maize variety resistance and leaf chemical constituents play a major role in shaping the phyllosphere fungal community. The work proposes a link between the assembled fungal communities within the phyllosphere with maize variety that is resistant to pathogenic fungi infection.
众所周知，植物基因型可在物种水平上调节叶际真菌。然而，关于植物品种如何塑造叶际真菌群落却知之甚少。在本研究中，对四种对[病原体名称缺失]具有不同抗性水平的玉米品种进行了高通量测序，以揭示影响叶际真菌群落组成的特性。所有四个玉米品种的优势真菌属在相对丰度上各不相同，其次是[第二个优势真菌属名称缺失]。层次聚类分析、非度量多维尺度分析和相似性分析证实，这四个品种叶际的真菌群落存在显著差异，并聚类到它们所栖息的各自玉米品种中。冗余分析（RDA）的结果表明，玉米抗性和叶片化学成分，包括氮、磷、单宁和黄酮类化合物，是决定叶际真菌群落组成的主要驱动因素。在这些因素中，发现玉米抗性影响最大，其次是磷。与其他玉米品种相比，高抗品种叶际真菌群落的共现网络具有更高的复杂性、完整性和稳定性。总之，玉米品种抗性和叶片化学成分在塑造叶际真菌群落中起主要作用。这项工作提出了叶际内组装的真菌群落与对病原真菌感染具有抗性的玉米品种之间的联系。
