小鼠乳腺导管内原位癌模型的建立。

Mouse intraductal modeling of primary ductal carcinoma in situ.

机构信息

Division of Molecular Pathology, The Netherlands Cancer Institute, 1066CX Amsterdam, The Netherlands; Oncode Institute, Amsterdam, The Netherlands.

Department of Pathology and Laboratory Medicine, The University of Kansas Medical Center, Kansas City, KS 66103, USA.

出版信息

STAR Protoc. 2023 Sep 15;4(3):102526. doi: 10.1016/j.xpro.2023.102526. Epub 2023 Aug 30.

DOI:10.1016/j.xpro.2023.102526

PMID:37651235

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10480770/

Abstract

Mouse intraductal modeling enables efficient in vivo propagation of pre-invasive breast cancer lesions and provides a suitable micro-environment for creating patient-derived tumor xenograft models of estrogen-receptor-positive breast cancer. Here, we present a protocol for mouse intraductal modeling of primary ductal carcinoma in situ (DCIS). We describe steps for processing primary DCIS tissues and performing intraductal injections. We then detail procedures for processing intraductal lesions for 3D whole-mount imaging or serial transplantation using magnetic bead sorting. For complete details on the use and execution of this protocol, please refer to Hutten et al. (2023)..

摘要

小鼠管内建模可有效促进乳腺原位癌前病变在体内的传播，并为建立雌激素受体阳性乳腺癌患者来源的肿瘤异种移植模型提供合适的微环境。本研究提供了一种用于原发性乳腺导管原位癌（DCIS）小鼠管内建模的方案。我们描述了处理原发性 DCIS 组织和进行管内注射的步骤。然后，我们详细介绍了用于 3D 整体成像或使用磁珠分选进行连续移植的管内病变处理程序。有关该方案使用和实施的完整详细信息，请参见 Hutten 等人（2023）。