Hsieh Jui-Chun, Chuang Shih-Te, Hsu Yu-Ting, Ho Shang-Tse, Li Kuan-Yi, Chou Shih-Hsuan, Chen Ming-Ju
Department of Animal Science and Technology, National Taiwan University, Taipei City, Taiwan.
Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung City, Taiwan.
Front Vet Sci. 2023 Aug 17;10:1228086. doi: 10.3389/fvets.2023.1228086. eCollection 2023.
There are differences in the gut microbiome and metabolome when the host undergoes different physical or pathological conditions. However, the inter-relationship of microbiome and metabolome biomarkers to potentially promote the health of dairy cows needs to be studied. Further, the development of next-generation probiotics for dairy cattle health promotion has not been demonstrated.
In the present study, we identified the microbiome and metabolome biomarkers associated with healthy cows.
We analyzed the relationships of the ruminal microorganism profile and metabolites between healthy and mastitis lactating dairy cows. The roles of bacterial biomarker were further verified by in vitro fermentation and cow-to-mouse fecal microbiota transplantation (FMT).
Two species, and subsp. , and six rumen metabolites were positively correlated with healthy cows by Spearman's correlation analysis. Through in vitro ruminal fermentation, inoculating and subsp. showed the upregulation of the levels of putrescine, xanthurenic acid, and pyridoxal in the mastitis ruminal fluid, which confirmed the inter-relationships between these microbiota and metabolites associated with healthy cows. Further, we verified the role of and subsp. in promoting health by FMT. The administration of and subsp. reduced the death rate and recovered the bodyweight loss of germ-free mice caused by FMT mastitis feces.
We provided evidence that the bacterial biomarkers alter downstream metabolites. This could indirectly indicate that the two bacterial biomarkers have the potential to be used as next-generation probiotics for dairy cattle, although it needs more evidence to support our hypothesis. Two species, and subsp. , with three metabolites, putrescine, xanthurenic acid, and pyridoxal, identified in the ruminal fluid, may point to a new health-promoting and disease-preventing approach for dairy cattle.
当宿主处于不同的生理或病理状态时，肠道微生物群和代谢组会存在差异。然而，微生物群和代谢组生物标志物之间的相互关系对促进奶牛健康的潜在作用仍有待研究。此外，尚未证实用于促进奶牛健康的下一代益生菌的开发。
在本研究中，我们鉴定了与健康奶牛相关的微生物群和代谢组生物标志物。
我们分析了健康和患乳腺炎的泌乳奶牛瘤胃微生物谱与代谢物之间的关系。通过体外发酵和牛到小鼠的粪便微生物群移植（FMT）进一步验证了细菌生物标志物的作用。
通过Spearman相关性分析，两种菌，即[具体菌种1]和[具体菌种2]亚种，以及六种瘤胃代谢物与健康奶牛呈正相关。通过体外瘤胃发酵，接种[具体菌种1]和[具体菌种2]亚种显示乳腺炎瘤胃液中腐胺、黄尿酸和吡哆醛水平上调，这证实了这些微生物群与健康奶牛相关代谢物之间的相互关系。此外，我们通过FMT验证了[具体菌种1]和[具体菌种2]亚种在促进健康方面的作用。给予[具体菌种1]和[具体菌种2]亚种可降低无菌小鼠因FMT乳腺炎粪便导致的死亡率，并恢复体重减轻。
我们提供了证据表明细菌生物标志物会改变下游代谢物。这可能间接表明这两种细菌生物标志物有潜力用作奶牛的下一代益生菌，尽管需要更多证据来支持我们的假设。在瘤胃液中鉴定出的两种菌，即[具体菌种1]和[具体菌种2]亚种，以及三种代谢物，腐胺、黄尿酸和吡哆醛，可能为奶牛指出一种新的促进健康和预防疾病的方法。
