The effects of temperature on nickel bioaccumulation and toxicity in the freshwater snail Lymnaea stagnalis.

It is well known that temperature can have important effects on the toxicity of metals (and other contaminants) to aquatic organisms. To date, research has mostly focused on thermal effects on acute metal toxicity, and there is a data gap on thermal effects on chronic metal toxicity to sensitive organisms that are particularly relevant to environmental risk assessment. This latter research is especially needed in the context of increased global temperature and heat waves frequency associated with climate change. We investigated temperature effects on chronic nickel (Ni) bioaccumulation and toxicity to the metal-sensitive freshwater snail Lymnaea stagnalis. In the laboratory, we conducted a series of experiments with juvenile snails that were pre-acclimated to different temperatures since their embryonic stage. We found that temperature and nickel separately had strong effects on juvenile growth rate and survival. Rising temperature from 18 to 26 °C had no noticeable effect on Ni-induced growth inhibition and Ni bioaccumulation in juvenile L. stagnalis exposed over 40 days to 0, 30 and 60 μg L of dissolved Ni. These results agreed with estimates of Ni uptake and elimination rates (k and k), which were either unaffected by temperature or increased by similar factors from 18 to 26 °C. On the other hand, a temperature increase from 18 to 26 °C appeared to exacerbate Ni lethality to juvenile snails in the 40-day toxicity test. This exacerbation might have been due to a combination of factors, including detrimental changes in metabolically available Ni pools and/or to sensitization of the organism under sub-optimal temperatures. Overall, our study shows that thermal effects on metal chronic toxicity are complex, with effects that can be response-specific and not directly related to metal toxicokinetic.