Department of Neurology, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemoon-gu, Seoul, 03722, South Korea.
Integrative Research Institute for Cerebrovascular and Cardiovascular Diseases, Yonsei University College of Medicine, Seoul, South Korea.
Sci Rep. 2023 Sep 4;13(1):14568. doi: 10.1038/s41598-023-41383-z.
Clot perviousness on computerized tomography (CT) is predictive of response to reperfusion therapy. This study aimed to determine the association of clot perviousness with ultrastructural features of clot in stroke patients undergoing endovascular thrombectomy. We quantitatively analyzed the ultrastructural components identified using scanning electron microscopy. The clot components were determined in the inner portions of the clots. Clot perviousness was assessed as thrombus attenuation increase (TAI) using noncontrast CT and CT angiography. We compared the association between the identified ultrastructural components and clot perviousness. The proportion of pores consisted of 3.5% on scanning electron microscopy images. The proportion of porosity in the inner portion was 2.5%. Among the ultrastructural components, polyhedrocytes were most commonly observed. The mean TAI was 9.3 ± 10.0 (median 5.6, interquartile range 1.1-14.3) Hounsfield units. TAI correlated positively with inner porosity (r = 0.422, p = 0.020). Among the ultrastructural clot components, TAI was independently associated with polyhedrocytes (B = - 0.134, SE = 0.051, p = 0.008). Clot perviousness is associated with porosity and the proportion of polyhdrocytes of clots.
计算机断层扫描（CT）显示的血栓通透性与再灌注治疗的反应相关。本研究旨在确定血管内血栓切除术治疗的卒中患者血栓的通透性与血栓超微结构特征之间的关系。我们使用扫描电子显微镜对血栓的超微结构成分进行了定量分析。确定了血栓内层的血栓成分。采用非增强 CT 和 CT 血管造影评估血栓通透性，定义为血栓衰减增加（TAI）。我们比较了确定的超微结构成分与血栓通透性之间的关联。扫描电镜图像上的孔隙率比例为 3.5%。血栓内层的孔隙率为 2.5%。在超微结构成分中，多角形细胞最常见。平均 TAI 为 9.3±10.0（中位数 5.6，四分位间距 1.1-14.3）HU。TAI 与内层孔隙率呈正相关（r=0.422，p=0.020）。在超微结构的血栓成分中，TAI 与多角形细胞独立相关（B=-0.134，SE=0.051，p=0.008）。血栓通透性与血栓的孔隙率和多角形细胞的比例有关。
