Juni E, Heym G A, Avery M
Appl Environ Microbiol. 1986 Sep;52(3):546-51. doi: 10.1128/aem.52.3.546-551.1986.
A defined medium for growth of 24 strains of Moraxella (Branhamella) catarrhalis was devised. This medium (medium B4) contains sodium lactate as a partial carbon source, proline as both a partial carbon source and a partial nitrogen source, aspartate as a partial nitrogen source, and the growth factors arginine, glycine, and methionine. Either aspartate, glutamate, or proline could serve as sole nitrogen source, but growth occurred at a significantly better rate if proline was present together with either aspartate or glutamate, or with both aspartate and glutamate. With the exception of strain ATCC 23246, all the strains had an absolute requirement for arginine and either a partial or absolute requirement for glycine. The concentration of glycine required for optimal growth was found to be relatively high for an amino acid growth factor. Heart infusion broth was found to be growth inhibitory for spontaneous mutants of one strain able to grow in the absence of arginine, and such mutants reverted readily to arginine dependence accompanied by the ability to grow faster on the complex medium. Growth rates in the defined medium B4 were enhanced by the simultaneous addition of asparagine, glutamate, glutamine, leucine, lysine, histidine, and phenylalanine.
设计了一种用于24株卡他莫拉菌（布兰汉菌）生长的限定培养基。这种培养基（B4培养基）含有乳酸钠作为部分碳源、脯氨酸作为部分碳源和部分氮源、天冬氨酸作为部分氮源，以及生长因子精氨酸、甘氨酸和蛋氨酸。天冬氨酸、谷氨酸或脯氨酸都可以作为唯一氮源，但如果脯氨酸与天冬氨酸或谷氨酸，或与天冬氨酸和谷氨酸同时存在，生长速度会明显加快。除ATCC 23246菌株外，所有菌株都绝对需要精氨酸，并且对甘氨酸有部分或绝对需求。对于一种氨基酸生长因子而言，发现最佳生长所需的甘氨酸浓度相对较高。发现心脏浸液肉汤对一株能够在无精氨酸条件下生长的自发突变体具有生长抑制作用，并且这种突变体很容易恢复为对精氨酸的依赖性，同时在复合培养基上生长得更快。同时添加天冬酰胺、谷氨酸、谷氨酰胺、亮氨酸、赖氨酸、组氨酸和苯丙氨酸可提高在限定培养基B4中的生长速度。
