Department of Biological Sciences, North Dakota State University, Fargo, ND, 58105, USA.
Department of Microbiological Sciences, North Dakota State University, Fargo, ND, 58105, USA.
Microb Ecol. 2023 Nov;86(4):2949-2958. doi: 10.1007/s00248-023-02296-6. Epub 2023 Sep 7.
Glyphosate is the most commonly used agricultural herbicide in the world. In aquatic ecosystems, glyphosate often adsorbs to benthic substrates or is metabolized and degraded by microorganisms. The effects of glyphosate on microbial communities vary widely as microorganisms respond differently to exposure. To help understand the impacts of glyphosate on the sediment microbiome, we conducted a microcosm experiment examining the responses of benthic sediment microbial communities to herbicide treatments. Sediments from a prairie pothole wetland were collected, and 16S rRNA gene sequencing was used to analyze community composition 2-h and 14-days after a single treatment of low (0.07 ppm), medium (0.7 ppm), or high (7 ppm) glyphosate, aminomethylphosphonic acid (glyphosate metabolite), or a glyphosate-based commercial formula. We found no significant differences in microbial community composition across treatments, concentration levels, or day of sampling. These findings suggest that microbial species in the Prairie Pothole Region of North America may be tolerant to glyphosate exposure.
草甘膦是世界上使用最广泛的农业除草剂。在水生生态系统中，草甘膦通常会被底栖基质吸附，或被微生物代谢和降解。由于微生物对暴露的反应不同，草甘膦对微生物群落的影响差异很大。为了帮助了解草甘膦对沉积物微生物组的影响，我们进行了一项微宇宙实验，研究了除草剂处理对底栖沉积物微生物群落的响应。从草原洼地湿地采集沉积物，在单次低（0.07ppm）、中（0.7ppm）、高（7ppm）草甘膦、氨甲基膦酸（草甘膦代谢物）或基于草甘膦的商业配方处理后 2 小时和 14 天，使用 16S rRNA 基因测序分析群落组成。我们发现处理、浓度水平或采样日之间的微生物群落组成没有显著差异。这些发现表明，北美草原洼地地区的微生物物种可能对草甘膦暴露具有耐受性。
