Zhou Zhimo, Zhang Yujie, Liu Meixun, Jia Wen, Cheng Ruyue, Shen Xi, He Fang
West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
Wei Sheng Yan Jiu. 2023 Jul;52(4):585-590. doi: 10.19813/j.cnki.weishengyanjiu.2023.04.011.
To explore the effects of different transplantation frequencies and time of fecal microbiota transplantation on mice.
Twenty-four C57BL/6J mice were randomly divided into control group, fecal microbiota transplantation group 1(FMT1), fecal microbiota transplantation group 2(FMT2), and fecal microbiota transplantation group 3(FMT3). The control group was used as the donor of fecal microbiota transplantation, and the FMT1, FMT2, and FMT3 groups were intervened with mixed antibiotics(200 μL/d) for 2 weeks, and received fecal bacterial suspension(200 μL/d). The transplantation time of the FMT1 group frequency was 1 time/d for 1 weeks, the FMT2 group was 1 time/d for 2 weeks, and the FMT3 group was 3 times/week for 2 weeks. At the end of the experiment, the feces of the mice were collected to analyze the gut microbiota.
Compared with the control group, there were more independent Amplicon Sequence Variants in the intestinal microbiota of mice in FMT1 group, FMT2 group and FMT3 group, and the ACE index and Chao1 index were significantly reduced(P<0.05). Beta diversity showed differences between fecal microbiota transplantation and control groups, with FMT2 and control groups being the closest. At the phylum level, there were two species in FMT1 group and one species in FMT3 group showed statistically significant differences compared with control group(P<0.05). However, there was no significant difference between the FMT2 group and the control group. At the genus level, there were 6 species in the FMT1 with statistically significant differences from the control group(P<0.05), and 2 species in the FMT2, 5 species in the FMT3 respectively. Among which FMT2 group has the least number of species that differed from the control group, suggesting that the compitsition of its intestinal microbiota is closet to that of the control group.
Fecal bacteria transplantation helps to restore the intestinal microbiota structure of mice cleaned by antibiotics, and different transplantation frequencies and transplantation times have different recovery effects on the intestinal microbiota of mice pretreated with antibiotics, and the fecal bacteria transplantation effect is better with 1 time/d lasting 2 weeks.
探讨不同移植频率和时间的粪便微生物群移植对小鼠的影响。
将24只C57BL/6J小鼠随机分为对照组、粪便微生物群移植组1（FMT1）、粪便微生物群移植组2（FMT2）和粪便微生物群移植组3（FMT3）。对照组作为粪便微生物群移植的供体，FMT1、FMT2和FMT3组用混合抗生素（200 μL/d）干预2周，然后接受粪便细菌悬液（200 μL/d）。FMT1组移植频率为每天1次，持续1周；FMT2组为每天1次，持续2周；FMT3组为每周3次，持续2周。实验结束时，收集小鼠粪便分析肠道微生物群。
与对照组相比，FMT1组、FMT2组和FMT3组小鼠肠道微生物群中独立的扩增子序列变异更多，ACE指数和Chao1指数显著降低（P<0.05）。β多样性显示粪便微生物群移植组与对照组之间存在差异，其中FMT2组与对照组最接近。在门水平上，FMT1组有两个物种、FMT3组有一个物种与对照组相比有统计学显著差异（P<0.05）。然而，FMT2组与对照组之间无显著差异。在属水平上，FMT1组有6个物种与对照组有统计学显著差异（P<0.05），FMT2组有2个物种，FMT3组有5个物种。其中FMT2组与对照组不同的物种数量最少，表明其肠道微生物群组成与对照组最接近。
粪便细菌移植有助于恢复经抗生素清除后的小鼠肠道微生物群结构，不同的移植频率和移植时间对经抗生素预处理的小鼠肠道微生物群有不同的恢复效果，每天1次持续2周的粪便细菌移植效果更佳。
