Gullberg U, Widegren B, Arnason U, Egesten A, Olsson I
Biochem Biophys Res Commun. 1986 Sep 30;139(3):1239-42. doi: 10.1016/s0006-291x(86)80310-2.
The eosinophil cationic protein (ECP) is a specific cytotoxic constituent of granules. In this work we demonstrated that ECP has a ribonuclease activity. Purified ECP was resolved by ion exchange chromatography into subfractions, which all showed ribonuclease activity. Another eosinophil granule protein, EPX, identical with eosinophil-derived neurotoxin (EDN) had a 125-fold higher RNase activity than ECP. ECP may exert its cytotoxic effects on parasites and cells because of its extreme basicity alone or it may be internalized and act by degrading mRNA.
嗜酸性粒细胞阳离子蛋白（ECP）是颗粒的一种特异性细胞毒性成分。在本研究中，我们证明ECP具有核糖核酸酶活性。通过离子交换色谱法将纯化的ECP分离成亚组分，所有亚组分均显示出核糖核酸酶活性。另一种嗜酸性粒细胞颗粒蛋白EPX，与嗜酸性粒细胞衍生的神经毒素（EDN）相同，其核糖核酸酶活性比ECP高125倍。ECP可能仅因其极高的碱性就对寄生虫和细胞发挥细胞毒性作用，或者它可能被内化并通过降解mRNA发挥作用。
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