A surface multiple imprinting layers membrane with well-oriented recognition sites for selective separation of chlorogenic acid from Ficus carica L.

Chlorogenic acid (CGA), known as an important natural antioxidative compound in Ficus carica L, has valuable application prospects on health food, functional food, nutrition and dietary formulations. In this study, a surface multiple imprinting layers membrane (SMILM) was developed and applied to separate CGA from F. carica. Two different imprinting layers were integrated onto the membrane surface in sequence. The first imprinting layer was formed by dopamine polymerization and the second imprinting layer was fabricated by atom transfer radical polymerization (ATRP) and boronate affinity. The prepared SMILM with well-oriented multiple recognition sites exhibited high adsorption capacity (52.08 mg·g in 60 min) for CGA and specific selectivity with imprinting factor (IF) of 3.06. Furthermore, the recognition mechanism of SMILM was clarified by molecular simulation and NMR. The SMILM was successfully applied to separate CGA from the fruits, peels and leaves of F. carica with recoveries of 90.22, 83.31 and 84.95 %, respectively.