Key Laboratory for Humid Subtropical Eco-geographical Processes of the Ministry of Education, Fujian Normal University, Fuzhou 350117, China.
School of Geographical Sciences, Fujian Normal University, Fuzhou 350117, China.
Ying Yong Sheng Tai Xue Bao. 2023 Jun;34(6):1547-1554. doi: 10.13287/j.1001-9332.202306.017.
Root-associated fungi play a vital role in maintaining nutrient absorption and health of host plants. To compare the responses of root-associated fungal community structures to nitrogen (N) and/or phosphorus (P) additions across differential mycorrhizal types, we collected roots of nine plant species belonging to three mycorrhizal types (arbuscular mycorrhiza, ectomycorrhiza, and ericoid mycorrhiza) under control and N and/or P addition treatments from a subtropical forest, and detected the diversity and community composition of fungi inhabiting roots through the high-throughput sequencing technique. The results showed that root-associated fungal communities of all nine plant species were mainly composed of Basidiomycota and Ascomycota. The relative abundance of Ascomycota and Basidiomycota was significantly lower and higher under the P addition than that under control, respectively. The relative abundance of Ascomycota of ericoid mycorrhizal trees was significantly higher than those of arbuscular mycorrhizal and ectomycorrhizal trees, while the relative abundance of Basidiomycota was significantly lower than the other two mycorrhizal types. Compared with the control, P addition significantly reduced the α-diversity and changed community composition of root-associated fungi across different mycorrhizal plant types, while no effect of N addition or mycorrhizal type was observed. Compared with the control and N addition treatments, NP addition caused root-associated fungal communities of all plants becoming integrally divergent. In addition, the fungal communities of ectomycorrhizal mycorrhizal trees became apparently convergent in comparison with those of arbuscular and ericoid mycorrhizal trees under the NP addition. Collectively, our results highlighted that P was a critical factor influencing community structures of tree root-associated fungi in subtropical forest soils. This study would enhance our understanding of the responses and maintenance mechanisms of plant root-associated fungal diversity under global environmental changes in the subtropical region.
根相关真菌在维持宿主植物的养分吸收和健康方面发挥着至关重要的作用。为了比较不同菌根类型下氮（N）和/或磷（P）添加对根相关真菌群落结构的响应，我们从亚热带森林中采集了属于三种菌根类型（丛枝菌根、外生菌根和杜鹃花菌根）的九种植物的根，在对照和 N 和/或 P 添加处理下，通过高通量测序技术检测了根内真菌的多样性和群落组成。结果表明，所有九种植物的根相关真菌群落主要由担子菌门和子囊菌门组成。与对照相比，P 添加下子囊菌门和担子菌门的相对丰度分别显著降低和升高。杜鹃花菌根树木的子囊菌门相对丰度显著高于丛枝菌根和外生菌根树木，而担子菌门的相对丰度显著低于其他两种菌根类型。与对照相比，P 添加显著降低了不同菌根植物类型根相关真菌的 α-多样性并改变了其群落组成，而 N 添加或菌根类型则没有影响。与对照和 N 添加处理相比，NP 添加导致所有植物的根相关真菌群落整体发生分歧。此外，与对照和 N 添加处理相比，NP 添加使外生菌根树木的真菌群落与丛枝菌根和杜鹃花菌根树木的真菌群落明显趋同。总之，我们的研究结果强调了 P 是影响亚热带森林土壤中树木根相关真菌群落结构的关键因素。本研究将提高我们对亚热带地区全球环境变化下植物根相关真菌多样性响应和维持机制的理解。
