Wuhan Fourth Hospital, Wuhan, Hubei 430032, China.
Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan, Hubei 430068, China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 3):126828. doi: 10.1016/j.ijbiomac.2023.126828. Epub 2023 Sep 9.
Tea polysaccharide conjugates (TPC) were used as fillers in the form of biopolymer or colloidal particles (TPC stabilized nanoemulsion, NE) for reinforcing alginate (ALG) beads to improve the probiotic viability. Results demonstrated that adding TPC or NE to ALG beads significantly enhanced the gastrointestinal viability of encapsulated probiotics when compared to free cells. Moreover, the survivability of free and ALG encapsulated probiotics markedly decreased to 2.03 ± 0.05 and 2.26 ± 0.24 log CFU/g, respectively, after 2 weeks ambient storage, indicating pure ALG encapsulation had no effective storage protective capability. However, adding TPC or NE could greatly enhance the ambient storage viability of probiotics, with ALG + NE beads possessing the best protection (8.93 ± 0.06 log CFU/g) due to their lower water activity and reduced porosity. These results suggest that TPC and NE reinforced ALG beads have the potential to encapsulate, protect and colonic delivery of probiotics.
茶多糖缀合物（TPC）被用作生物聚合物或胶体颗粒（TPC 稳定的纳米乳液，NE）的填充物，用于增强海藻酸钠（ALG）珠粒以提高益生菌的存活率。结果表明，与游离细胞相比，向 ALG 珠粒中添加 TPC 或 NE 可显著提高包封益生菌的胃肠道存活率。此外，游离和 ALG 包封益生菌的存活率在 2 周环境储存后分别显著下降至 2.03±0.05 和 2.26±0.24 log CFU/g，表明纯 ALG 包封没有有效的储存保护能力。然而，添加 TPC 或 NE 可以大大提高益生菌的环境储存存活率，其中 ALG+NE 珠粒具有最佳的保护作用（8.93±0.06 log CFU/g），因为它们具有较低的水活度和减少的孔隙率。这些结果表明，TPC 和 NE 增强的 ALG 珠粒具有包封、保护和结肠递送益生菌的潜力。
