一种测量雌性果蝇性接受度的实验方案。
A protocol for measuring the sexual receptivity of female Drosophila.
机构信息
State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China; Sino-Danish College, University of Chinese Academy of Sciences, Beijing 101408, China; Department of Drug Design and Pharmacology, University of Copenhagen, 2100 Copenhagen, Denmark; Sino-Danish Center for Education and Research, Beijing 101408, China.
ENT Institute and Department of Otorhinolaryngology, Eye & ENT Hospital, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan University, Shanghai 200031, China.
出版信息
STAR Protoc. 2023 Dec 15;4(4):102563. doi: 10.1016/j.xpro.2023.102563. Epub 2023 Sep 12.
Female receptivity in mating is crucial for successful copulation, but protocols for quantifying female behaviors reflecting receptivity are scarce compared to the analysis of male behaviors. Here, we present a protocol for assessing the sexual receptivity of female Drosophila that considers behaviors from both sexes. We describe steps for preparing and loading flies into a courtship chamber, video recording the behaviors of the pairs, and analyzing their behavioral displays. This protocol includes behavior recognition criteria suitable for typical laboratory settings. For complete details on the use and execution of this protocol, please refer to Yang et al. (2023)..
在交配中，雌性的接受能力对于成功交配至关重要，但与分析雄性行为相比，用于量化反映雌性接受能力的行为的方案却很少。在这里，我们提出了一种评估雌性果蝇性接受能力的方案，该方案考虑了两性的行为。我们描述了准备和将果蝇装入求偶室、视频记录对的行为以及分析它们的行为表现的步骤。该方案包括适合典型实验室环境的行为识别标准。有关此方案的使用和执行的完整详细信息，请参阅 Yang 等人。（2023 年）。
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