College of Forestry, Guizhou University, Guiyang, China.
Pol J Microbiol. 2023 Sep 20;72(3):325-337. doi: 10.33073/pjm-2023-034. eCollection 2023 Sep 1.
is the primary pathogen of blueberry root rot; furthermore, we found that can also cause root rot in blueberries. spp. is widely used to control plant diseases. We isolated (TM11) from blueberry rhizosphere soil to explore its control effect and mechanism on and . We found that the inhibitory effects of TM11 volatiles and broth metabolites on were significant, but only volatile metabolites had a significant inhibitory effect on its growth. Twelve known antimicrobial metabolites were detected from the methanol extract of TM11 fermentation broth by HPLC-MS. TM11 lysed and coiled around the hyphae of and . The pot experiment showed that TM11 had significant control effects against and , and inoculation of TM11 prior to that of and was more effective. The TM11, TM11 and , or and distilled water treatments had different effects on the activities of superoxide dismutase, peroxidase and catalase, and the enzyme activity levels exhibited the following order: TM11 > TM11 and or > distilled water. The results showed that TM11 provided effective control of blueberry root rot.
是蓝莓根腐病的主要病原体；此外，我们发现 也会引起蓝莓根腐病。 spp. 被广泛用于防治植物病害。我们从蓝莓根际土壤中分离出 （TM11），以探索其对 和 的控制效果和机制。我们发现 TM11 挥发物和培养液代谢物对 的抑制作用显著，但只有 TM11 挥发性代谢物对其生长有显著抑制作用。通过 HPLC-MS 从 TM11 发酵液的甲醇提取物中检测到 12 种已知的抗菌代谢物。TM11 裂解并缠绕在 和 的菌丝上。盆栽试验表明，TM11 对 和 具有显著的防治效果，且在 和 接种前接种 TM11 更为有效。TM11、TM11 和 或 和 蒸馏水处理对超氧化物歧化酶、过氧化物酶和过氧化氢酶的活性有不同的影响，酶活性水平的顺序为：TM11 > TM11 和 或 > 蒸馏水。结果表明，TM11 为蓝莓根腐病提供了有效的防治。
