Department of Gynecology and Obstetrics, Savur Prof Dr Aziz Sancar District State Hospital, Mardin, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Erciyes University, 38039, Kayseri, Turkey.
Biol Trace Elem Res. 2024 Jun;202(6):2730-2743. doi: 10.1007/s12011-023-03871-1. Epub 2023 Sep 25.
Ovarian ischemia is a gynecological emergency that occurs as a result of ovarian torsion, affects women of reproductive age, and reduces ovarian reserve. The current study was designed to investigate the effect of boric acid taken in different ways on histopathological changes, autophagy, oxidative stress, and DNA damage caused by ischemia and reperfusion in the ovary of adult female rats. We established seven groups of 70 adult female rats: untreated control, intraperitoneal boric acid group (IpBA), oral boric acid group (OBA), ischemia/reperfusion group (ischemia/2 h reperfusion; OIR), ischemia/reperfusion and local boric acid group (OIR + LBA), ischemia/reperfusion and intraperitoneal boric acid group (OIR + IpBA), and ischemia/reperfusion and oral boric acid group (OIR + OBA). On the 31st day of the experimental procedure, both ovaries were harvested for histologic (hematoxylen and eosin and Masson trichrom), biochemical (ELISA and AMH, MDA, SOD, and CAT analyses), and comet evaluation. In the OIR group, hemorrhage, edema, inflammation, and diminished follicle reserve were seen in the ovary. Boric acid treatment reduced the ovarian ischemia/reperfusion damage, and the follicles exhibited similar morphological features to the control group. Moreover, boric acid treatment decreased the levels of Hsp70, NF-KB, COX-2, and CD31, which increased as a result of OIR. On the other hand, SCF and AMH levels, which decreased as a result of OIR, increased with boric acid treatment. The levels of autophagy markers (Beclin-1, LC3, and p62) reached values close to those of the control group. According to the biochemical findings, it was concluded that boric acid is also effective on oxidative stress, and the AMH level was particularly high in the OIR + OBA group, consistent with the immunohistochemical staining result. In addition, it was observed that the DNA damage caused by OIR reached values close to those of the control group, especially in the OBA after OIR. This study showed the therapeutic effects of boric acid on OIR injuries; thus, boric acid may be a potential therapeutic agent for ovarian protection and fertility preservation in cases that may cause ovarian torsion.
卵巢缺血是一种妇科急症，由卵巢扭转引起，影响育龄妇女，减少卵巢储备。本研究旨在探讨不同途径给予硼酸对成年雌性大鼠卵巢缺血再灌注引起的组织病理学变化、自噬、氧化应激和 DNA 损伤的影响。我们建立了 7 组 70 只成年雌性大鼠：未处理对照组、腹腔内硼酸组（IpBA）、口服硼酸组（OBA）、缺血/再灌注组（缺血/2 h 再灌注；OIR）、局部硼酸组（OIR+LBA）、腹腔内硼酸组（OIR+IpBA）和口服硼酸组（OIR+OBA）。在实验程序的第 31 天，采集双侧卵巢进行组织学（苏木精和伊红和 Masson 三色）、生化（ELISA 和 AMH、MDA、SOD 和 CAT 分析）和彗星评估。在 OIR 组中，卵巢可见出血、水肿、炎症和卵泡储备减少。硼酸治疗减轻了卵巢缺血/再灌注损伤，卵泡表现出与对照组相似的形态特征。此外，硼酸治疗降低了由于 OIR 而增加的 Hsp70、NF-KB、COX-2 和 CD31 的水平。另一方面，由于 OIR 而降低的 SCF 和 AMH 水平随着硼酸治疗而增加。自噬标志物（Beclin-1、LC3 和 p62）的水平接近对照组。根据生化发现，硼酸对氧化应激也有效，OIR+OBA 组的 AMH 水平特别高，与免疫组化染色结果一致。此外，观察到 OIR 引起的 DNA 损伤接近对照组，尤其是 OIR 后 OBA。这项研究表明硼酸对 OIR 损伤具有治疗作用；因此，硼酸可能是一种有潜力的治疗药物，可用于保护卵巢和保存生育能力，以防发生卵巢扭转。
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