Du Yujie, Zhu Jinfan, Tian Zhonghuan, Long Chaoan
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, National R&D Center for Citrus Preservation, National Centre of Citrus Breeding, Huazhong Agricultural University, Wuhan 430070, China.
Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Wuhan 430070, China.
J Fungi (Basel). 2023 Sep 18;9(9):941. doi: 10.3390/jof9090941.
has become one of the main pathogens in citrus due to its high spore production and easy spread. In this study, the function of the APSES transcription factor in was characterized, and the results indicated that it was involved in conidium and conidiophore development. No conidiophores were observed in the mycelium of the ∆ mutant that had grown for two days, while an abnormal conidiophore was found after another two days of incubation, and only small thin phialides as well as a very small number of spores were formed at the top of the hyphae. Moreover, it was observed that the ∆ mutant showed various defects, such as reduced hydrophobicity and decreased tolerance to cell wall inhibitors and HO. Compared to the original , the colony diameter of the ∆ mutant was not significantly affected, but the growth of aerial hyphae was obviously induced. In in vivo experiments, the spore production of the ∆ mutant grown on citrus fruit was remarkably decreased; however, there was no significant difference in the lesion diameter between the mutant and original strain. It could be inferred that less spore production might result in reduced spread in citrus, thereby reducing the green mold infection in citrus fruit during storage. This study provided a gene, , which played key role in the sporulation of , and the results might provide a reference for the molecular mechanisms of sporulation in .
由于其高孢子产量和易于传播，已成为柑橘中的主要病原体之一。在本研究中，对柑橘绿霉病菌中APSES转录因子的功能进行了表征，结果表明它参与分生孢子和分生孢子梗的发育。在生长两天的∆突变体菌丝体中未观察到分生孢子梗，而在再培养两天后发现了异常的分生孢子梗，并且在菌丝顶端仅形成了小而细的瓶梗以及极少数的孢子。此外，观察到∆突变体表现出各种缺陷，例如疏水性降低以及对细胞壁抑制剂和HO的耐受性降低。与原始菌株相比，∆突变体的菌落直径没有受到显著影响，但气生菌丝的生长明显受到诱导。在体内实验中，在柑橘果实上生长的∆突变体的孢子产量显著降低；然而，突变体与原始菌株之间的病斑直径没有显著差异。可以推断，较少的孢子产量可能导致在柑橘中的传播减少，从而减少柑橘果实在储存期间的绿霉病感染。本研究提供了一个在柑橘绿霉病菌孢子形成中起关键作用的基因，该结果可能为柑橘绿霉病菌孢子形成的分子机制提供参考。
