Saizen Ayana, Stipkovits Letitia, Muto Yukiyo, Serventi Luca
Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand.
Foods. 2023 Sep 15;12(18):3447. doi: 10.3390/foods12183447.
Peanuts contain nutritionally relevant levels of protein, yet are poorly digestible. Fermentation is a promising technique to boost legume protein quality, but its effect on the protein quality of raw peanuts has not been investigated. This study aimed to assess the impact of fermentation on the in vitro protein digestibility and free amino acid profile of cooked peanut slurry (peanut to water ratio 1:1). Cultures used were subsp. and a commercial fresh cheese culture that contained subsp. , , , and , fermenting at 38 °C for 48 h. Samples fermented with the combination of cultures showed higher protein digestibility, as well as softer texture. Significant increases were observed only in the sample fermented with the fresh cheese culture. While the fresh cheese culture improved the free amino acid profile after fermentation, the combination of the cultures decreased all free amino acid concentrations except for glutamine, alanine, and proline. The observed increases in in vitro protein digestibility and the free amino acid profile may be attributed to the proteolytic activities of the cultures.
花生含有营养相关水平的蛋白质,但消化性较差。发酵是提高豆类蛋白质质量的一种有前景的技术,但尚未研究其对生花生蛋白质质量的影响。本研究旨在评估发酵对煮熟花生浆(花生与水比例为1:1)的体外蛋白质消化率和游离氨基酸谱的影响。使用的培养物是亚种以及一种含有亚种、、、和的商业新鲜奶酪培养物,在38℃下发酵48小时。用混合培养物发酵的样品显示出更高的蛋白质消化率以及更柔软的质地。仅在用新鲜奶酪培养物发酵的样品中观察到显著增加。虽然新鲜奶酪培养物在发酵后改善了游离氨基酸谱,但混合培养物降低了除谷氨酰胺、丙氨酸和脯氨酸之外的所有游离氨基酸浓度。观察到的体外蛋白质消化率和游离氨基酸谱的增加可能归因于培养物的蛋白水解活性。