College of Animal Science, Jilin University, Changchun 130062, China.
Genes (Basel). 2023 Sep 4;14(9):1759. doi: 10.3390/genes14091759.
Mink embryos enter a period of diapause after the embryo develops into the blastocyst, and its reactivation is mainly caused by an increase in polyamine. The specific process of embryo diapause regulation and reactivation remains largely unexamined. This study aimed to identify changes in metabolites in the early pregnancy of mink by comparing and analyzing in serum metabolites up to twenty-nine days after mating. Blood samples were taken on the first day of mating, once a week until the fifth week. Metabolomic profiles of the serum samples taken during this period were analyzed by ultra-performance liquid chromatography/mass spectrometry. Multivariate statistical analyses identified differential metabolite expression at different time points in both positive and negative ion modes. The levels of dopamine, tyramine, L-phenylalanine, L-tyrosine, tyrosine, L-kynurenine, L-lysine, L-arginine, D-ornithine, and leucine changed significantly. These metabolites may be associated with the process of embryo diapause and subsequent reactivation.
水貂胚胎在发育成囊胚后进入休眠期，其重新激活主要是由于多胺的增加。胚胎休眠调节和重新激活的具体过程在很大程度上仍未得到研究。本研究旨在通过比较和分析交配后 29 天内血清代谢物，来鉴定水貂早期妊娠的代谢物变化。在交配的第一天、第五周前的每周一次采集血液样本。通过超高效液相色谱/质谱分析在此期间采集的血清样本的代谢组学图谱。在正离子和负离子模式下，多变量统计分析确定了不同时间点的差异代谢物表达。多巴胺、酪胺、L-苯丙氨酸、L-酪氨酸、酪氨酸、L-犬尿氨酸、L-赖氨酸、L-精氨酸、D-鸟氨酸和亮氨酸的水平发生了显著变化。这些代谢物可能与胚胎休眠和随后的重新激活过程有关。
