Wu Chung-Hsin, Pan Xun-Sheng, Su Li-Yu, Yang Shieh-Yueh
School of Life Science, National Taiwan Normal University, Taipei 106, Taiwan.
Zaixing Animal Hospital, Taipei 116, Taiwan.
Int J Mol Sci. 2023 Sep 6;24(18):13771. doi: 10.3390/ijms241813771.
The number of elderly dogs is increasing significantly worldwide, and many elderly dogs develop canine cognitive dysfunction syndrome (CCDS). CCDS is the canine analog of Alzheimer's disease (AD) in humans. It is very important to develop techniques for detecting CDDS in dogs. Thus, we used the detection of neurofilament light chains (NfL) in plasma as a blood-based biomarker for the early diagnosis of canine Alzheimer's disease using immunomagnetic reduction (IMR) technology by immobilizing NfL antibodies on magnetic nanoparticles. According to the 50-point CCDS rating scale, we divided 36 dogs into 15 with CCDS and 21 without the disease. The results of our IMR assay showed that the plasma NfL levels of dogs with CCDS were significantly increased compared to normal dogs ( < 0.01). By plasma biochemical analysis, we further confirmed that the liver and renal dysfunction biomarkers of dogs with CCDS were significantly elevated compared to normal dogs ( < 0.01-0.05). On the basis of our preliminary study, we propose that IMR technology could be an ideal biosensor for detecting plasma NfL for the early diagnosis of CCDS.
全球老年犬的数量正在显著增加，许多老年犬会患上犬类认知功能障碍综合征（CCDS）。CCDS是人类阿尔茨海默病（AD）在犬类中的类似病症。开发检测犬类CDDS的技术非常重要。因此，我们通过将神经丝轻链（NfL）抗体固定在磁性纳米颗粒上，利用免疫磁珠法（IMR）技术检测血浆中的NfL，将其作为犬类阿尔茨海默病早期诊断的一种基于血液的生物标志物。根据50分的CCDS评分量表，我们将36只犬分为15只患有CCDS的犬和21只未患该病的犬。我们的IMR检测结果表明，与正常犬相比，患有CCDS的犬血浆NfL水平显著升高（<0.01）。通过血浆生化分析，我们进一步证实，与正常犬相比，患有CCDS的犬的肝脏和肾脏功能障碍生物标志物显著升高（<0.01 - 0.05）。基于我们的初步研究，我们提出IMR技术可能是一种用于检测血浆NfL以早期诊断CCDS的理想生物传感器。
