卵形疟原虫的形态异型性和分子特征：巴西亚马逊地区暴发疫情的发现。
Morphological atypia and molecular profile of Plasmodium vivax: Findings from an outbreak in the Brazilian Amazon.
机构信息
Graduate Program in Biology of Infectious and Parasitic Agents, Federal University of Pará, Rua Augusto Corrêa, 01, Belém, Pará, Brazil - Laboratory of Malaria Entomology, Parasitology Section, Evandro Chagas Institute, Rodovia BR 316, Km 7, Ananindeua, Pará, Brazil.
Laboratory of Basic Research in Malaria, Parasitology Section, Evandro Chagas Institute, Rodovia BR 316, Km 7, Ananindeua, Pará, Brazil.
出版信息
Parasite. 2023;30:38. doi: 10.1051/parasite/2023039. Epub 2023 Sep 29.
This study aimed to perform morphological and molecular analyses of parasites isolated from the blood of malaria-infected individuals during an outbreak in the Microregion of Cametá, State of Pará, Brazilian Amazon. A total of 260 positive samples were identified by microscopy as Plasmodium vivax; however, in three samples, forms considered unusual for the species were found and defined as morphological atypia of P. vivax. Single P. vivax infection was confirmed by qPCR in all samples. Among 256 genotyped samples, the VK247 genotype alone was identified in 255 samples, and the VK210 genotype was found in only one. The study showed that this malaria outbreak was caused by the etiological agent P. vivax, and for the first time, morphological atypia was described in isolates circulating in Brazil. Likewise, for the first time, the VK247 genotype was detected predominantly in single infections in an area of the State of Pará, which may suggest a greater circulation of the genotype in the region.
本研究旨在对巴西亚马逊州卡梅塔微区疟疾流行期间从疟疾病例血液中分离出的寄生虫进行形态学和分子分析。共有 260 份显微镜检查阳性样本被鉴定为间日疟原虫，但在 3 份样本中发现了被认为是该物种异常的形态，被定义为间日疟原虫形态异型性。所有样本均通过 qPCR 确认了单纯间日疟原虫感染。在 256 份基因分型样本中，255 份样本单独鉴定出 VK247 基因型，仅在 1 份样本中发现 VK210 基因型。研究表明，此次疟疾暴发是由间日疟原虫引起的，这是首次在巴西流行的寄生虫分离株中描述形态异型性。同样，首次在帕拉州一个地区检测到 VK247 基因型主要存在于单纯感染中，这可能表明该基因型在该地区的传播更为广泛。
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