原癌基因c-mos在未分化畸胎瘤细胞中的表达。

Expression of c-mos proto-oncogene in undifferentiated teratocarcinoma cells.

作者信息

Ogiso Y, Matsumoto M, Morita T, Nishino H, Iwashima A, Matsushiro A

出版信息

Biochem Biophys Res Commun. 1986 Oct 30;140(2):477-84. doi: 10.1016/0006-291x(86)90757-6.

Abstract

Proto-oncogene c-mos, the cellular homologue of the transforming gene of Moloney murine sarcoma virus, has been characterized by the lack of expression in a variety of differentiated tissues, possibly because of the existence of an inhibitory upstream sequence. We detected mos-related transcripts in undifferentiated embryonal carcinoma cells of pluripotential cell line 311. The sizes of three major transcripts detected were estimated to be 1.8, 4.6 and 6.1-kilobases (kb) by northern analysis. Furthermore, these transcriptions were suppressed when the cell differentiation was induced by retinoic acid. Taken together, the results suggest that mos product plays a role in early stages of development.

摘要

原癌基因c-mos是莫洛尼鼠肉瘤病毒转化基因的细胞同源物，其特点是在多种分化组织中缺乏表达，这可能是由于存在一个抑制性上游序列。我们在多能细胞系311的未分化胚胎癌细胞中检测到了与mos相关的转录本。通过Northern分析，检测到的三种主要转录本的大小估计分别为1.8、4.6和6.1千碱基（kb）。此外，当用视黄酸诱导细胞分化时，这些转录会受到抑制。综合来看，这些结果表明mos产物在发育的早期阶段发挥作用。