Department of Aquaculture, Institute for Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, 2100, Godollo, Hungary.
Balaton Fish Management Non-Profit Ltd, Horgony U. 1., 8600, Siofok, Hungary.
Fish Physiol Biochem. 2024 Oct;50(5):2001-2012. doi: 10.1007/s10695-023-01245-x. Epub 2023 Oct 3.
The aim of our study was to determine the efficacy of utilizing cryopreserved common carp sperm (in comparison to fresh sperm) for propagation at a Hungarian aquaculture facility. The sperm was frozen in 5 mL straws using an extender method that was previously tested in common carp. Sperm motility was monitored using a computer-assisted sperm analysis system. The hatching and malformation rates among the specimens were recorded before the stocking of larvae in both groups. The growth (body weight, total length) and survival rates of the fish were measured during the pre-nursing (from May to June: between 1 and 26 days post hatching) and grow-out periods (from June to October: between 26 and 105 days post hatching) of the same year. The fresh sperm, which was collected and pooled prior to fertilization, showed high MOT (97%), pMOT (92%), VCL (106 µm s), LIN (75%), and ALH (1.84 µm). Prior to the fertilization trial of the cryopreserved sperm, low MOT (34%), pMOT (14%), and VCL (61 µm s) values were observed in frozen-thawed sperm. A significantly higher hatching rate was measured in the fresh sperm group (87%) when compared to the cryopreserved sperm group (42%). No significant difference in the overall malformation rate was observed in larvae originating from either the fresh or frozen sperm. A significant difference between the two test groups was observed in the incidence of deformed tails (fresh: 20%, cryopreserved: 55%). Except for one sampling period, no significant difference in the body weight and total length of the fish larvae was found between the two groups throughout the pre-nursing and grow-out periods. A significantly higher larvae survival rate was noted in the fresh sperm (72%) as compared to the cryopreserved group (43%) by the end of the pre-nursing stage. However, no significant difference in survival rate was observed for the cryopreserved sperm (96%) in comparison to the fresh sperm (95%) by the end of the grow-out stage. The results of this study showed, for the first time in large-scale pond culturing, an equal growth and viability in larvae propagated from cryopreserved sperm when compared to fresh sperm (despite the limited available rearing ponds provided by the commercial company).
我们的研究目的是确定在匈牙利水产养殖设施中利用冷冻保存的鲤鱼精子（与新鲜精子相比）进行繁殖的效果。精子在 5ml straws 中用之前在鲤鱼中测试过的 extender 方法冷冻。使用计算机辅助精子分析系统监测精子活力。在两组幼虫放养前，记录标本的孵化率和畸形率。在同一年的预护理（从 5 月到 6 月：孵化后 1 至 26 天）和生长（从 6 月到 10 月：孵化后 26 至 105 天）期间，测量鱼的生长（体重、全长）和成活率。在受精前收集和混合的新鲜精子显示出高 MOT（97%）、pMOT（92%）、VCL（106µm/s）、LIN（75%）和 ALH（1.84µm）。在冷冻保存精子的受精试验之前，冷冻解冻精子的 MOT（34%）、pMOT（14%）和 VCL（61µm/s）值较低。与冷冻保存精子组（42%）相比，新鲜精子组的孵化率（87%）显著更高。来自新鲜或冷冻精子的幼虫的总体畸形率没有显著差异。在两个测试组中，畸形尾的发生率存在显著差异（新鲜：20%，冷冻：55%）。除了一个采样期外，在整个预护理和生长期间，两组鱼幼虫的体重和全长均无显著差异。在预护理阶段结束时，新鲜精子（72%）的幼虫成活率明显高于冷冻精子组（43%）。然而，在生长阶段结束时，冷冻精子（96%）的成活率与新鲜精子（95%）相比没有显著差异。这项研究的结果首次表明，在大规模池塘养殖中，与新鲜精子相比，从冷冻保存的精子中繁殖的幼虫的生长和活力是相等的（尽管商业公司提供的可用养殖池塘有限）。
