Malik Rohit, Kalra Sunishtha, Singh Govind, Gahlot Vinod, Kajal Anjali
Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak, Haryana, India; Gurugram Global College of Pharmacy, Gurugram, India.
Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak, Haryana, India.
Brain Res. 2024 Jan 1;1822:148616. doi: 10.1016/j.brainres.2023.148616. Epub 2023 Oct 2.
The goal of this research study was to see how plant extracts of Acorus calamus Linn. and Cordia dichotoma G. Forst. overcome scopolamine-induced Alzheimer's type dementia in mice by activating the cholinergic system, anti-oxidant and protection of neuronal death in the brain (hippocampus region). Scopolamine (1 mg/kg i.p.) reduced mice's routine in behavioral parameters such as Morris Water Maze (MWM), Elevated Plus Maze (EPM), and also the locomotor activity. It also decreases antioxidant levels such as Reduced glutathione (GSH) and also Superoxide dismutase (SOD) but also increases the level of Acetylcholinesterase enzyme (AChE) in brain. Assessment of various behavioral, and biochemical parameters (AChE, SOD, GSH, and Nitrite level) were compared with each group. Acorus calamus (hydro-alcoholic 1:1) 600 mg/kg p.o. and the combination (Acorus calamus 600 mg/kg p.o. + Cordia dichotoma 750 mg/kg p.o.) group showed significant results as compared to Cordia dichotoma 750 mg/kg p.o.in behavioral as well as in biochemical parameters. Histological studies showed significant neuroprotection in the Acorus calamus-treated group and the combination-treated groups. In the future, the Acorus calamus and the combination are possibly helpful in the treatment of various cognitive disorders or it may be valuable to investigate the pharmacological potential of such plant extracts during the treatment of neurodegenerative disorders.
本研究的目的是观察菖蒲（Acorus calamus Linn.）和破布木（Cordia dichotoma G. Forst.）的植物提取物如何通过激活胆碱能系统、抗氧化以及保护大脑（海马区）神经元死亡来克服东莨菪碱诱导的小鼠阿尔茨海默型痴呆。东莨菪碱（1毫克/千克腹腔注射）降低了小鼠在行为参数方面的表现，如莫里斯水迷宫（MWM）、高架十字迷宫（EPM），以及运动活性。它还降低了抗氧化剂水平，如还原型谷胱甘肽（GSH）和超氧化物歧化酶（SOD），但同时增加了大脑中乙酰胆碱酯酶（AChE）的水平。对每组的各种行为和生化参数（AChE、SOD、GSH和亚硝酸盐水平）进行了比较。菖蒲（水醇1:1）600毫克/千克口服给药组以及联合用药组（菖蒲600毫克/千克口服给药 + 破布木750毫克/千克口服给药）在行为和生化参数方面与破布木750毫克/千克口服给药组相比显示出显著效果。组织学研究表明，菖蒲治疗组和联合治疗组具有显著的神经保护作用。未来，菖蒲及其联合用药可能有助于治疗各种认知障碍，或者在神经退行性疾病治疗过程中研究此类植物提取物的药理潜力可能具有重要价值。
