中国食源性及临床沙门氏菌肠炎血清型 Typhimurium 及其单相变异株的喹诺酮类耐药特征。
Characterization of quinolone resistance in Salmonella enterica serovar Typhimurium and its monophasic variants from food and patients in China.
机构信息
Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China.
Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China.
出版信息
J Glob Antimicrob Resist. 2023 Dec;35:216-222. doi: 10.1016/j.jgar.2023.09.010. Epub 2023 Oct 4.
OBJECTIVES
The study aimed to characterize the quinolone resistance of Salmonella enterica serovar Typhimurium and its monophasic variant (Salmonella enterica serovar 1,4,[5],12:i:-) isolated from food and patients in China.
METHODS
All of the isolates were assessed for quinolone susceptibility via the broth microdilution method. Then, the isolates were checked for mutations within quinolone resistance-determining regions of gyrA, gyrB, parC, and parE and were examined for plasmid-mediated quinolone resistance genes.
RESULTS
High rates of resistance to nalidixic acid in the S. Typhimurium (70.7%) and S. 1,4,[5],12:i:- (41.9%) isolates were observed, and a considerable proportion of isolates with reduced susceptibility to ciprofloxacin and levofloxacin were also detected. The high frequency of mutations in GyrA (60.8%) and a variety of genes (aac[6']-Ib-cr [23.2%], oqxAB [19.2%], qnrS [13.6%], and qnrA [3.2%]) conferring quinolone resistance in these Salmonella isolates were noteworthy. Lastly, the isolates carrying qnrS for transferability and transmission of the quinolone resistance were analysed by conjugation. Multiple locus variable-number tandem repeat analysis profiles indicated that some qnrS-positive isolates were clonally related, whilst the other isolates were genetically divergent. This suggested that both clonal spread of resistant strains and horizontal transmission of the plasmid-mediated resistance genes contributed to the dissemination of qnrS-positive Salmonella isolates.
CONCLUSION
This study highlights the prevalence of quinolone-resistant S. Typhimurium and S. 1,4,[5],12:i:- in China, posing a threat to public health.
目的
本研究旨在描述中国食品和患者中分离出的肠炎沙门氏菌血清型 Typhimurium 及其单相变体（肠炎沙门氏菌血清型 1,4,[5],12:i:-）的喹诺酮耐药性特征。
方法
采用肉汤微量稀释法评估所有分离株的喹诺酮药敏性。然后，检查喹诺酮耐药决定区（gyrA、gyrB、parC 和 parE）中的突变情况，并检查质粒介导的喹诺酮耐药基因。
结果
肠炎沙门氏菌（70.7%）和肠炎沙门氏菌血清型 1,4,[5],12:i:-（41.9%）分离株对萘啶酸的耐药率较高，同时还检测到相当比例的对环丙沙星和左氧氟沙星敏感性降低的分离株。这些沙门氏菌分离株中 GyrA（60.8%）的高频突变和多种基因（aac[6']-Ib-cr [23.2%]、oqxAB [19.2%]、qnrS [13.6%]和 qnrA [3.2%]）的存在表明这些分离株具有喹诺酮耐药性。最后，通过接合试验分析了携带 qnrS 可转移和传播喹诺酮耐药性的分离株。多位点可变数串联重复分析谱表明，一些 qnrS 阳性分离株具有克隆相关性，而其他分离株则具有遗传差异。这表明耐药菌株的克隆传播和质粒介导的耐药基因的水平传播都导致了 qnrS 阳性沙门氏菌分离株的传播。
结论
本研究强调了中国普遍存在的喹诺酮耐药性肠炎沙门氏菌血清型 Typhimurium 和 S. 1,4,[5],12:i:-，这对公共卫生构成了威胁。
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