胃癌转移相关基因 NT5DC2 预示患者预后不良。

Gastric cancer metastasis-related NT5DC2 indicates unfavorable prognosis of patients.

机构信息

Department of Internal Medicine-Oncology, Inner Mongolia Autonomous Region People's Hospital, Hohhot, Inner Mongolia, P.R. China.

Department of Internal Medicine, Inner Mongolia Medical University, Hohhot, Inner Mongolia, P.R. China.

出版信息

Medicine (Baltimore). 2023 Oct 6;102(40):e35030. doi: 10.1097/MD.0000000000035030.

DOI:10.1097/MD.0000000000035030

PMID:37800836

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10553061/

Abstract

PURPOSE

Approximately 80 to 90% of patients with gastric cancer (GC) eventually develop into metastatic GC nowadays,because GC is difficult to be diagnosed at an early stage. GC patients with metastases typically have a poor prognosis. It is necessary to explore a potential prognostic marker in metastatic GC.

METHODS

All GC data were obtained from The Cancer Genome Atlas and Gene Expression Omnibus databases. The metastasis-related candidate gene and its role in GC were analyzed by comprehensive analysis.

RESULTS

Totally 1049 metastasis-related genes were identified in GC. Univariate Cox regression analysis screened the top 10 genes (PDHX, SLC43A1, CSAG2, NT5DC2, CSAG1, FMN1, MED1, HIVEP2, FNDC3A, and PPP1R2) that were closely correlated with prognosis of GC patients. Among which, NT5DC2 was screened as the target gene for subsequent study. The NT5DC2 expression were increased in primary GC and metastatic GC samples. Moreover, GC patients with high NT5DC2 expression exhibited shorter overall survival and post progression survival, and the NT5DC2 was metastatic GC patients' independent prognostic factor. Totally 29 pathways were activated in metastatic GC samples with high NT5DC2 expression. Four immune cells' infiltration were significantly different between NT5DC2 high and low expressed metastatic GC patients. NT5DC2 showed significantly negative correlations with 6 types of immune cells' critical marker genes and 5 types of immune cell infiltration. The 10 immune checkpoint expressions were decreased in high NTDC2 expression metastatic GC patients.

CONCLUSIONS

NT5DC2 plays a prognostic role in metastatic GC. GC patients with high NT5DC2 expression indicates unfavorable prognosis.

摘要

目的

如今，约 80%至 90%的胃癌（GC）患者最终发展为转移性 GC，因为 GC 在早期很难被诊断。转移性 GC 患者的预后通常较差。因此，有必要探索转移性 GC 的潜在预后标志物。

方法

所有 GC 数据均来自于癌症基因组图谱和基因表达综合分析数据库。通过综合分析，确定了与 GC 转移相关的候选基因及其在 GC 中的作用。

结果

共鉴定出 1049 个与 GC 转移相关的基因。单因素 Cox 回归分析筛选出与 GC 患者预后密切相关的前 10 个基因（PDHX、SLC43A1、CSAG2、NT5DC2、CSAG1、FMN1、MED1、HIVEP2、FNDC3A 和 PPP1R2）。其中，NT5DC2 被筛选为后续研究的靶基因。NT5DC2 在原发性 GC 和转移性 GC 样本中的表达均升高。此外，NT5DC2 高表达的 GC 患者总生存期和无进展生存期均较短，NT5DC2 是转移性 GC 患者的独立预后因素。在 NT5DC2 高表达的转移性 GC 样本中，共激活了 29 条通路。NT5DC2 高表达和低表达的转移性 GC 患者之间有 4 种免疫细胞浸润存在显著差异。NT5DC2 与 6 种免疫细胞关键标志物基因和 5 种免疫细胞浸润呈显著负相关。高 NT5DC2 表达的转移性 GC 患者中 10 种免疫检查点表达降低。