Yoneda Naru, Matoba Osamu, Saita Yusuke, Nomura Takanori
Opt Lett. 2023 Oct 15;48(20):5273-5276. doi: 10.1364/OL.496419.
Optical scanning holography (OSH) can be applied to 3D fluorescent imaging. However, the optical setup for OSH is complicated due to the requirement of a phase shifter, a 2D mechanical scanner, and an interferometer. Although motionless optical scanning holography (MOSH) can overcome the problem, quantitative phase imaging (QPI) has not yet been realized because MOSH can only obtain incoherent holograms. If QPI in MOSH is realized, MOSH can be applied to various applications. In this Letter, MOSH-based QPI (MOSH-QPI) is proposed. In addition, a simple description of a coherent mode of OSH is presented. In the proof-of-principle experiment, the spatially divided phase-shifting technique is applied to reduce the number of measurements. The feasibility of MOSH-QPI is confirmed by measuring a phase distribution of a microlens array. MOSH-QPI is also applied to measure practical samples, and its results are compared with the experimental results of the conventional one using a Mach-Zehnder interferometer.
光学扫描全息术（OSH）可应用于三维荧光成像。然而，由于需要一个移相器、一个二维机械扫描仪和一个干涉仪，OSH的光学设置很复杂。尽管静止光学扫描全息术（MOSH）可以克服这个问题，但由于MOSH只能获得非相干全息图，定量相位成像（QPI）尚未实现。如果在MOSH中实现了QPI，那么MOSH就可以应用于各种应用。在这封信中，提出了基于MOSH的QPI（MOSH-QPI）。此外，还给出了OSH相干模式的简单描述。在原理验证实验中，应用空间分割相移技术来减少测量次数。通过测量微透镜阵列的相位分布，证实了MOSH-QPI的可行性。MOSH-QPI也被应用于测量实际样品，并将其结果与使用马赫-曾德尔干涉仪的传统方法的实验结果进行比较。
