Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology Kattankulathur, 603 203, Chengalpattu, Tamil Nadu, India.
Department of Biotechnology, Faculty of Science and Humanities, SRM Institute of Science and Technology Kattankulathur, 603 203, Chengalpattu, Tamil Nadu, India.
J Ethnopharmacol. 2024 Jan 30;319(Pt 3):117314. doi: 10.1016/j.jep.2023.117314. Epub 2023 Oct 11.
Herbal medicines derived from plant extraction are affordable, more therapeutically effective, and have fewer side effects than contemporary medications. Vitex negundo L. (V. negundo). is a medicinal shrub, which contains numerous phytoconstituents. In ancient medicinal practices, V. negundo was primarily prescribed as an analgesic and anti-inflammatory drug.
This study aims to evaluate the anti-inflammatory and antioxidant characteristics of crude extracts from V. negundo leaves, including those derived from petroleum ether (P), methanol (M), and aqueous (A) solvents. Additionally, the research seeks to identify the specific bioactive compounds responsible for these observed properties.
The nitric oxide scavenging study was performed to evaluate the V. negundo crude extract's ability to function as a nitric oxide scavenger. Protein denaturation and proteinase inhibition experiments were employed to study the ability of extracts to suppress proteolysis and inhibit the enzymes that cause tissue injury. The membrane-stabilizing potency of plant extracts were examined through the process of heat-induced hemolysis. The ability of the extracts to neutralize free radicals showed a dose-dependent response, and the aqueous extract exhibited substantially higher activity in both FRAP and DPPH. The GC-MS analysis of V. negundo extracts revealed a vast array of pharmacologically active metabolites. Based on this Bioassay-guided fractionation approach, the optimal extract was selected for the potent molecule isolation and characterization.
The findings demonstrated that the aqueous extract of V. negundo exhibited markedly superior radical scavenging and anti-inflammatory capabilities compared to the other two extracts. Furthermore, a new molecule, 3,4,9-trimethyl-7-propyldecanoic acid was isolated from this extract, and its chemical structure was successfully determined.
This study revealed that the aqueous extract of V. negundo demonstrated notably stronger in vitro anti-inflammatory and antioxidant properties in comparison to the methanol and petroleum ether extracts. The identified active compound, 3,4,9-trimethyl-7-propyldecanoic acid is likely responsible for the extract's free radical scavenging and anti-inflammatory effects. Furthermore, conducting both in vitro and in vivo studies is crucial to substantiate the potential of this active constituent for the development of an anti-inflammatory drug derived from V. negundo.
植物药草提取的草药价格实惠，疗效更显著，副作用也比现代药物少。牡荆（Vitex negundo L.）是一种药用灌木，含有多种植物成分。在古代医学实践中，牡荆主要被用作镇痛药和消炎药。
本研究旨在评估牡荆叶的粗提取物（包括石油醚（P）、甲醇（M）和水（A）溶剂提取物）的抗炎和抗氧化特性，并确定具有这些观察到的特性的特定生物活性化合物。
通过测定牡荆粗提取物清除一氧化氮的能力，评估其作为一氧化氮清除剂的能力。采用蛋白变性和蛋白酶抑制实验研究提取物抑制蛋白水解和抑制引起组织损伤的酶的能力。通过热诱导溶血过程，研究植物提取物的膜稳定能力。提取物中和自由基的能力呈剂量依赖性反应，水提取物在 FRAP 和 DPPH 中均表现出更高的活性。牡荆提取物的 GC-MS 分析显示出大量具有药理活性的代谢物。基于这种基于生物测定的分级分离方法，选择最佳提取物进行有效分子的分离和鉴定。
结果表明，牡荆水提取物表现出比其他两种提取物更显著的自由基清除和抗炎能力。此外，从该提取物中分离出一种新分子，3,4,9-三甲基-7-丙基癸酸，并成功确定了其化学结构。
本研究表明，与甲醇和石油醚提取物相比，牡荆水提取物在体外表现出更强的抗炎和抗氧化特性。鉴定出的活性化合物 3,4,9-三甲基-7-丙基癸酸可能是提取物清除自由基和抗炎作用的原因。此外，进行体外和体内研究对于证实该活性成分开发牡荆抗炎药物的潜力至关重要。
