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重组人肿瘤坏死因子检测的标准化方法及参考建议。

Proposal of standardized methods and reference for assaying recombinant human tumor necrosis factor.

作者信息

Yamazaki S, Onishi E, Enami K, Natori K, Kohase M, Sakamoto H, Tanouchi M, Hayashi H

出版信息

Jpn J Med Sci Biol. 1986 Jun;39(3):105-18. doi: 10.7883/yoken1952.39.105.

DOI:10.7883/yoken1952.39.105
PMID:3784109
Abstract

Two assay methods for recombinant human tumor necrosis factor (rH-TNF) were developed, one a biological L-cell assay and the other an enzyme-linked immunosorbent assay. The accuracy and reproducibility of each and the correlation between the two were studied. As a result of this investigation, the two assay methods were found appropriate for standardization of rH-TNF. A freeze-dried reference was prepared, and examination of its potency and stability showed it to be suitable for use as a reference standard for rH-TNF assays.

摘要

开发了两种重组人肿瘤坏死因子（rH-TNF）的检测方法，一种是生物L细胞检测法，另一种是酶联免疫吸附检测法。研究了每种方法的准确性和重现性以及两者之间的相关性。通过这项研究，发现这两种检测方法适用于rH-TNF的标准化。制备了冻干参考品，对其效价和稳定性的检测表明它适合用作rH-TNF检测的参考标准品。

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Proposal of standardized methods and reference for assaying recombinant human tumor necrosis factor.重组人肿瘤坏死因子检测的标准化方法及参考建议。
Jpn J Med Sci Biol. 1986 Jun;39(3):105-18. doi: 10.7883/yoken1952.39.105.
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A monoclonal antibody-based enzyme immunoassay for quantitation of human tumor necrosis factor binding protein I, a soluble fragment of the 60 kDa TNF receptor, in biological fluids.一种基于单克隆抗体的酶免疫测定法，用于定量检测生物体液中的人肿瘤坏死因子结合蛋白I（60 kDa肿瘤坏死因子受体的可溶性片段）。
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