Department of Biology, College of Science, United Arab Emirates University, Al Ain, 15551, United Arab Emirates.
Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON, K1A 0C6, Canada.
Sci Data. 2023 Oct 16;10(1):705. doi: 10.1038/s41597-023-02610-4.
In the United Arab Emirates, sudden decline syndrome (SDS) is a destructive disease of date palm caused by the soil-borne fungal pathogen Fusarium proliferatum (Fp) DSM106835. Here, a high-resolution genome assembly of Fp DSM106835 was generated using PacBio HiFi sequencing with Omni-C data to provide a high-quality chromatin-organised reference genome with 418 scaffolds, totalling 58,468,907 bp in length and an N50 value of 4,383,091 bp from which 15,580 genes and 16,321 transcripts were predicted. The assembly achieved a complete BUSCO score of 99.2% for 758 orthologous genes. Compared to seven other Fp strains, Fp DSM106835 exhibited the highest continuity with a cumulative size of 44.26 Mbp for the first ten scaffolds/contigs, surpassing the assemblies of all examined Fp strains. Our findings of the high-quality genome of Fp DSM106835 provide an important resource to investigate its genetics, biology and evolutionary history. This study also contributes to fulfill the gaps in fungal knowledge, particularly the genes/metabolites associated with pathogenicity during the plant-pathogen interaction responsible for SDS.
在阿拉伯联合酋长国，猝倒综合征（SDS）是一种破坏性的棕榈树疾病，由土壤传播的真菌病原体尖孢镰刀菌（Fp）DSM106835 引起。在这里，使用 PacBio HiFi 测序和 Omni-C 数据生成了 Fp DSM106835 的高分辨率基因组组装，提供了一个高质量的染色质组织参考基因组，共有 418 个支架，总长 58,468,907bp，N50 值为 4,383,091bp，从中预测了 15,580 个基因和 16,321 个转录本。该组装实现了 758 个同源基因的完整 BUSCO 评分 99.2%。与其他七个 Fp 菌株相比，Fp DSM106835 与前十个支架/连段的累积大小为 44.26 Mbp 的连续性最高，超过了所有检查的 Fp 菌株的组装。我们对 Fp DSM106835 的高质量基因组的研究提供了一个重要的资源，用于研究其遗传学、生物学和进化历史。这项研究还有助于填补真菌知识的空白，特别是与 SDS 中植物-病原体相互作用中致病性相关的基因/代谢物。
