OsCBL1 通过 OsCCA1 负调控 OsNRT2.2 来调节水稻氮利用效率。
OsCBL1 modulates rice nitrogen use efficiency via negative regulation of OsNRT2.2 by OsCCA1.
机构信息
Key Laboratory of Molecular Biology and Gene Engineering of Jiangxi Province, College of Life Science, Nanchang University, Nanchang, 330031, China.
Department of Chemistry, University of Kentucky, Lexington, KY, USA.
出版信息
BMC Plant Biol. 2023 Oct 18;23(1):502. doi: 10.1186/s12870-023-04520-4.
BACKGROUND
For cereal crop breeding, it is meaningful to improve utilization efficiency (NUE) under low nitrogen (LN) levels while maintaining crop yield. OsCBL1-knockdown (OsCBL1-KD) plants exhibited increased nitrogen accumulation and NUE in the field of low N level.
RESULTS
OsCBL1-knockdown (OsCBL1-KD) in rice increased the expression of a nitrate transporter gene OsNRT2.2. In addition, the expression of OsNRT2.2, was suppressed by OsCCA1, a negative regulator, which could directly bind to the MYB-binding elements (EE) in the region of OsNRT2.2 promoter. The OsCCA1 expression was found to be down-regulated in OsCBL1-KD plants. At the low Nitrogen (N) level field, the OsCBL1-KD plants exhibited a substantial accumulation of content and higher NUE, and their actual biomass remained approximately as the same as that of the wild type.
CONCLUSION
These results indicated that down-regulation of OsCBL1 expression could upregulate the expression of OsNRT2.2 by suppressing the expression of OsCCA1and then increasing the NUE of OsCBL1-KD plants under low nitrogen availability.
背景
对于谷类作物的培育，在维持产量的同时提高低氮水平下的利用效率（NUE）是有意义的。OsCBL1 敲低（OsCBL1-KD）植株在低氮水平下表现出氮积累和 NUE 的增加。
结果
水稻中 OsCBL1 的敲低（OsCBL1-KD）增加了硝酸盐转运基因 OsNRT2.2 的表达。此外，负调控因子 OsCCA1 可直接结合到 OsNRT2.2 启动子区域的 MYB 结合元件（EE）上，抑制 OsNRT2.2 的表达，而 OsCCA1 的表达在 OsCBL1-KD 植株中被下调。在低氮（N）水平的田间，OsCBL1-KD 植株表现出含量的大量积累和更高的 NUE，其实际生物量与野生型基本相同。
结论
这些结果表明，下调 OsCBL1 的表达可以通过抑制 OsCCA1 的表达而上调 OsNRT2.2 的表达，从而在低氮供应下提高 OsCBL1-KD 植株的 NUE。
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