Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, P. R. China.
Science Center for Future Foods, Jiangnan University, Wuxi 214122, P. R. China.
J Agric Food Chem. 2023 Nov 1;71(43):15926-15941. doi: 10.1021/acs.jafc.3c05074. Epub 2023 Oct 19.
To achieve an adequate sense of sweetness with a healthy low-sugar diet, it is necessary to explore and produce sugar alternatives. Recently, glycoside sweeteners and their biosynthetic approaches have attracted the attention of researchers. In this review, we first outlined the synthetic pathways of glycoside sweeteners, including the key enzymes and rate-limiting steps. Next, we reviewed the progress in engineered microorganisms producing glycoside sweeteners, including synthesis, whole-cell catalysis synthesis, and synthesis. The applications of metabolic engineering strategies, such as cofactor engineering and enzyme modification, in the optimization of glycoside sweetener biosynthesis were summarized. Finally, the prospects of combining enzyme engineering and machine learning strategies to enhance the production of glycoside sweeteners were discussed. This review provides a perspective on synthesizing glycoside sweeteners in microbial cells, theoretically guiding the bioproduction of glycoside sweeteners.
要在低糖饮食中获得足够的甜味感，就需要探索和生产糖替代品。最近，糖苷类甜味剂及其生物合成方法引起了研究人员的关注。在这篇综述中，我们首先概述了糖苷类甜味剂的合成途径，包括关键酶和限速步骤。接下来，我们回顾了工程微生物生产糖苷类甜味剂的进展，包括化学合成、全细胞催化合成和酶法合成。总结了代谢工程策略在糖苷类甜味剂生物合成优化中的应用，如辅酶工程和酶修饰。最后，讨论了将酶工程和机器学习策略相结合以提高糖苷类甜味剂产量的前景。本综述为在微生物细胞中合成糖苷类甜味剂提供了一个视角，从理论上指导了糖苷类甜味剂的生物生产。
