Plant Biology Graduate Program and Department of Biology, University of Massachusetts Amherst, Amherst, Massachusetts, USA.
College of Biology and Food Engineering, Innovation and Practice Base for Postdoctors, Anyang Institute of Technology, Anyang, China.
Mol Ecol. 2023 Nov;32(22):5971-5985. doi: 10.1111/mec.17172. Epub 2023 Oct 20.
Weedy rice (Oryza spp.) is a weedy relative of the cultivated rice that competes with the crop and causes significant production loss. The BHA (blackhull awned) US weedy rice group has evolved from aus cultivated rice and differs from its ancestors in several important weediness traits, including flowering time, plant height and seed shattering. Prior attempts to determine the genetic basis of weediness traits in plants using linkage mapping approaches have not often considered weed origins. However, the timing of divergence between crossed parents can affect the detection of quantitative trait loci (QTL) relevant to the evolution of weediness. Here, we used a QTL-seq approach that combines bulked segregant analysis and high-throughput whole genome resequencing to map the three important weediness traits in an F population derived from a cross between BHA weedy rice with an ancestral aus cultivar. We compared these QTLs with those previously detected in a cross of BHA with a more distantly related crop, indica. We identified multiple QTLs that overlapped with regions under selection during the evolution of weedy BHA rice and some candidate genes possibly underlying the evolution weediness traits in BHA. We showed that QTLs detected with ancestor-descendant crosses are more likely to be involved in the evolution of weediness traits than those detected from crosses of more diverged taxa.
杂草稻（Oryza spp.）是一种与栽培稻亲缘关系密切的杂草，与作物竞争并导致显著的产量损失。BHA（黑稃芒）美国杂草稻群体是由栽培稻进化而来的，与它的祖先在几个重要的杂草特征上存在差异，包括开花时间、株高和种子破碎性。以前使用连锁图谱方法来确定植物杂草特征的遗传基础的尝试，通常没有考虑杂草的起源。然而，杂交亲本之间的分歧时间可能会影响与杂草进化相关的数量性状基因座（QTL）的检测。在这里，我们使用了一种 QTL-seq 方法，该方法结合了混池分离分析和高通量全基因组重测序，以绘制来自 BHA 杂草稻与祖先 aus 栽培稻杂交产生的 F 群体的三个重要杂草特征的图谱。我们将这些 QTL 与以前在 BHA 与更远缘作物籼稻杂交中检测到的 QTL 进行了比较。我们鉴定了多个与 BHA 杂草稻进化过程中受选择影响的区域重叠的 QTL，以及一些可能是 BHA 杂草特征进化的候选基因。我们表明，与祖先后代杂交检测到的 QTL 比从更多分化的类群杂交检测到的 QTL 更有可能参与杂草特征的进化。
