College of Survey and Planning, Shangqiu Normal University, Shangqiu, China.
College of Biology and Food, Shangqiu Normal University, Shangqiu, China.
PeerJ. 2023 Oct 20;11:e16324. doi: 10.7717/peerj.16324. eCollection 2023.
Plant-pathogen interactions occur in the apoplast comprising the cell wall matrix and the fluid in the extracellular space outside the plasma membrane. However, little is known regarding the contribution of the apoplastic proteome to systemic acquired resistance (SAR).
Specifically, SAR was induced by inoculating plants with DC3000 avrRps4. The apoplast washing fluid (AWF) was collected from the systemic leaves of the SAR-induced or mock-treated plants. A label free quantitative proteomic analysis was performed to identified the proteins related to SAR in AWF.
A total of 117 proteins were designated as differentially accumulated proteins (DAPs), including numerous pathogenesis-related proteins, kinases, glycosyl hydrolases, and redox-related proteins. Functional enrichment analyses shown that these DAPs were mainly enriched in carbohydrate metabolic process, cell wall organization, hydrogen peroxide catabolic process, and positive regulation of catalytic activity. Comparative analysis of proteome data indicated that these DAPs were selectively enriched in the apoplast during the induction of SAR.
The findings of this study indicate the apoplastic proteome is involved in SAR. The data presented herein may be useful for future investigations on the molecular mechanism mediating the establishment of SAR.
植物-病原体相互作用发生在质外体中，质外体包括细胞壁基质和质膜外细胞外空间中的流体。然而，关于质外体蛋白质组对系统获得性抗性（SAR）的贡献知之甚少。
具体来说，通过用 DC3000 avrRps4 接种植物来诱导 SAR。从 SAR 诱导或模拟处理植物的系统叶片中收集质外体洗涤液（AWF）。进行无标记定量蛋白质组学分析，以鉴定与 AWF 中的 SAR 相关的蛋白质。
共鉴定出 117 种差异积累蛋白（DAP），包括许多与发病相关的蛋白、激酶、糖苷水解酶和氧化还原相关蛋白。功能富集分析表明，这些 DAP 主要富集在碳水化合物代谢过程、细胞壁组织、过氧化氢分解代谢过程和催化活性的正调控中。蛋白质组数据的比较分析表明，这些 DAP 在 SAR 诱导过程中选择性地富集在质外体中。
本研究的结果表明质外体蛋白质组参与 SAR。本文提供的数据可能有助于对介导 SAR 建立的分子机制的进一步研究。
