Department of Ophthalmology, Yozgat City Hospital, Yozgat, Turkey.
Department of Ophthalmology, Meram Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey.
Graefes Arch Clin Exp Ophthalmol. 2024 Mar;262(3):957-965. doi: 10.1007/s00417-023-06280-1. Epub 2023 Oct 25.
The aim of this study was to evaluate the expression of placental growth factor (PLGF), neuropilin-1 (NP-1), and neuropilin-2 (NP-2) molecules in primary pterygium tissue compared with normal conjunctival tissue.
The records of 42 patients who underwent excision surgery with autografts for primary pterygium (pterygium group) and 20 patients who underwent conjunctival nevus excision surgery (control group) in the same period were reviewed retrospectively. The samples obtained from the pterygium tissues in the pterygium group and the clean conjunctival tissues adjacent to the nevus in the control group were collected from the archive. Immunohistochemical stains of the primary antibodies-1/100 diluted PLGF, NP-1, and NP-2 (Abcam Cambridge Science Park, UK)-were applied to all groups. Staining intensities and the percentage of positive cells in epithelial, endothelial, stromal, and inflammatory cells were analyzed by an experienced pathologist.
The positivity rates of PLGF and NP-2 expression in epithelial, endothelial, stromal, and inflammatory cells were found to be higher in the pterygium group than in the control group (PLGF: p < 0.001, p < 0.001, p = 0.001, and p < 0.001, respectively; NP-2: p < 0.001 for all). Staining intensities for PLGF and NP-2 were higher in the pterygium group than in the control group (PLGF: p < 0.001, p < 0.001, p = 0.005, and p < 0.001, respectively; NP-2: p < 0.001, p < 0.001, p = 0.001, and p < 0.001, respectively). However, no significant differences were found in any cell type in terms of NP-1 expression positivity rates (p = 0.730, p = 0.121, p = 0.524, and p = 0.624, respectively) or staining intensity (p = 0.716, p = 0.147, p = 0.147, and p = 0.780, respectively).
PLGF and NP-2 levels were found to be higher in pterygium tissue, while there was no difference in NP-1. These results indicate the possible roles of NP-2 and PLGF in primary pterygium.
本研究旨在比较原发性翼状胬肉组织与正常结膜组织中胎盘生长因子（PLGF）、神经纤毛蛋白-1（NP-1）和神经纤毛蛋白-2（NP-2）分子的表达。
回顾性分析了 42 例因原发性翼状胬肉行自体移植切除术患者（翼状胬肉组）和同期 20 例行结膜色素痣切除术患者（对照组）的病历。从翼状胬肉组的翼状胬肉组织和对照组的色素痣周围的清洁结膜组织中采集样本，并从存档中获取。用一抗（Abcam Cambridge Science Park，UK 稀释 1/100 倍的 PLGF、NP-1 和 NP-2）进行免疫组织化学染色。由一位经验丰富的病理学家分析上皮细胞、内皮细胞、基质细胞和炎症细胞的染色强度和阳性细胞百分比。
与对照组相比，翼状胬肉组上皮细胞、内皮细胞、基质细胞和炎症细胞中 PLGF 和 NP-2 的阳性率更高（PLGF：p<0.001，p<0.001，p=0.001，p<0.001，分别；NP-2：均为 p<0.001）。翼状胬肉组的 PLGF 和 NP-2 染色强度高于对照组（PLGF：p<0.001，p<0.001，p=0.005，p<0.001，分别；NP-2：均为 p<0.001）。然而，在任何细胞类型中，NP-1 的阳性率（p=0.730，p=0.121，p=0.524，p=0.624，分别）或染色强度（p=0.716，p=0.147，p=0.147，p=0.780，分别）均无显著差异。
在翼状胬肉组织中发现 PLGF 和 NP-2 水平升高，而 NP-1 则无差异。这些结果表明 NP-2 和 PLGF 可能在原发性翼状胬肉中发挥作用。
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