Department of Internal Medicine, Member of the German Center for Lung Research (DZL), Justus Liebig University of Giessen, Giessen, Germany.
Department of Internal Medicine, Member of the German Center for Lung Research (DZL), Justus Liebig University of Giessen, Giessen, Germany.
STAR Protoc. 2023 Dec 15;4(4):102660. doi: 10.1016/j.xpro.2023.102660. Epub 2023 Oct 25.
Right ventricular failure (RVF) is the leading cause of death in patients with pulmonary hypertension. Here, we present a protocol for pulmonary artery banding in mice to generate a model of pressure-overload-induced RVF. We describe steps for anesthesia of mice, endotracheal intubation, and pulmonary artery banding surgery. We then detail procedures for phenotyping and analysis. Our approach does not involve complete blockage of the pulmonary flow during clip placement and is, therefore, associated with low intraoperative mortality. For complete details on the use and execution of this protocol, please refer to Veith et al. (2020)..
右心衰竭（RVF）是肺动脉高压患者死亡的主要原因。在这里，我们提供了一种在小鼠中进行肺动脉环扎术以产生压力超负荷诱导的 RVF 模型的方案。我们描述了麻醉、气管内插管和肺动脉环扎手术的步骤。然后，我们详细介绍了表型分析和分析的步骤。我们的方法在放置夹时不会完全阻塞肺血流，因此术中死亡率较低。如需详细了解本方案的使用和执行情况，请参阅 Veith 等人（2020 年）。
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