基于非靶向代谢组学研究藏猪动脉粥样硬化发生过程中的代谢产物特征
Characteristics of Metabolites in the Development of Atherosclerosis in Tibetan Minipigs Determined Using Untargeted Metabolomics.
机构信息
Academy of Chinese Medicine & Institute of Comparative Medicine, Zhejiang Chinese Medical University, Hangzhou 310024, China.
Key Laboratory of Systems Health Science of Zhejiang Province, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Hangzhou 310024, China.
出版信息
Nutrients. 2023 Oct 18;15(20):4425. doi: 10.3390/nu15204425.
BACKGROUND
Atherosclerosis (AS) is a chronic progressive disease caused by lipometabolic disorder. However, the pathological characteristics and mechanism of AS have not been fully clarified. Through high-fat and high-cholesterol diet induction, Tibetan minipigs can be used as the AS model animals, as they have a very similar AS pathogenesis to humans.
METHODS
In this study, we built an AS model of Tibetan minipigs and identified the differential abundance metabolites in the development of AS based on untargeted metabolomics.
RESULTS
We found that sphingolipid metabolism and glucose oxidation were obviously higher in the AS group and phenylalanine metabolism was reduced in the AS group. Moreover, in the development of AS, gluconolactone was enriched in the late stage of AS whereas biopterin was enriched in the early stage of AS.
CONCLUSIONS
Our research provides novel clues to investigate the metabolic mechanism of AS from the perspective of metabolomics.
背景
动脉粥样硬化（AS）是一种由脂代谢紊乱引起的慢性进行性疾病。然而，AS 的病理特征和机制尚未完全阐明。通过高脂肪和高胆固醇饮食诱导，藏猪可用作 AS 模型动物，因为它们与人类的 AS 发病机制非常相似。
方法
本研究通过非靶向代谢组学方法，建立了藏猪 AS 模型，并鉴定了 AS 发生发展过程中的差异丰度代谢物。
结果
我们发现，在 AS 组中，鞘脂代谢和葡萄糖氧化明显升高，而苯丙氨酸代谢降低。此外，在 AS 的发生发展过程中，晚期 AS 中富集了葡庚糖酸内酯，而早期 AS 中则富集了生物蝶呤。
结论
我们的研究从代谢组学的角度为研究 AS 的代谢机制提供了新的线索。
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