胚胎期鸡心房心肌细胞聚集体中的复极化电流
Repolarization currents in embryonic chick atrial heart cell aggregates.
作者信息
Shrier A, Clay J R
出版信息
Biophys J. 1986 Nov;50(5):861-74. doi: 10.1016/S0006-3495(86)83527-5.
Abstract
Outward membrane currents in aggregates of atrial cells prepared from 7-12-d-old chick embryonic hearts were measured with the two microelectrode voltage-clamp technique. Two outward current components, Ix1 and Ix2, were found in the plateau potential range of the action potential. The Ix1 component is activated between -50 and -20 mV; the Ix2 component is activated between -15 and +20 mV. The Ix1 component inwardly rectifies, whereas Ix2 has an approximately linear current-voltage relation. These preparations lack a time-dependent pacemaker current component, even though they beat spontaneously with an interbeat interval of approximately 1 s. A mathematical model of electrical activity is described based on our measurements of time-dependent outward current, and measurements in the literature of inward current components.
摘要
采用双微电极电压钳技术测量了从7至12日龄鸡胚心脏制备的心房细胞聚集体的外向膜电流。在动作电位的平台期范围内发现了两个外向电流成分，即Ix1和Ix2。Ix1成分在-50至-20 mV之间被激活；Ix2成分在-15至+20 mV之间被激活。Ix1成分向内整流，而Ix2具有近似线性的电流-电压关系。这些制剂缺乏时间依赖性的起搏电流成分，尽管它们以约1秒的心动周期间隔自发搏动。基于我们对时间依赖性外向电流的测量以及文献中内向电流成分的测量，描述了一个电活动的数学模型。
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